兴义鸭肌肉生长抑制素基因多态性与屠宰性状的相关性分析

Abstract

Abstract: In order to understand the relationship between single nucleotide polymorphism sites(SNPs)of myostatin(MSTN) gene and slaughter traits in Xingyi duck, MSTN gene was used as a candidate gene for slaughter traits. The polymorphisms of the exon of MSTN gene in Xingyi duck were detected by direct sequencing of PCR products and gene cloning. The results showed that there were 8 single nucleotide polymorphism sites (SNPs) in 60 Xingyi ducks, there were 5 mutations in exon 1 of SNP1(G77A), SNP2(A91G), SNP3(G130A), SNP4 (C325T), SNP5(C331T), there was no mutation in exon 2, and there were 3 mutations in exon 3 of SNP6(C206T), SNP7 (A235G), SNP8 (C256A). By correlation analysis between the 8 SNPs and slaughter traits, it showed that there were no significant associations between the genetype and slaughter traits of Xingyi ducks (P>0.05). The result of this study could enrich MSTN gene research data and provide reference for duck breeding.

Key words: MSTN gene; Xingyi duck; slaughter traits

CLC Number:

S813.3

PENG Bang-xing, LI Hui, YI Heng-jie, ZHAO Zhong-hai. Polymorphisms of MSTN Gene and its Relationship with Slaughter Traits in Xingyi Duck[J]. , 2014, 41(12): 225-230.

References

1 马现永,施振旦,曹永长,等. 鸡、鹅肌肉生成抑制因子基因的克隆及序列分析[J]. 华南农业大学学报, 2004,25(2):85～88.
2 中华人民共和国农业部. NY/T 823-2004.家禽生产性能名词术语和度量统计方法[M]. 北京:中国农业出版社,2004.
3 卢俊清,黄苇,赵楠,等. 北京鸭肌肉生长抑制素基因多态与屠体性状关系分析[J].中国畜牧兽医, 2008, 35(10):61～63.
4 刘倩,陈永华,蔡凤仙,等. 高邮鸭生长抑制素基因外显子3多态性与腹脂率的关联分析[J].中国家禽, 2012, 34(23):27～30.
5 刘晓琴,马喜山,唐中林,等. 猪MSTN基因的多态性和生长性状关联分析[J].畜牧与兽医,2013,44(7):1063～1069.
6 易恒洁,李辉,杨胜林,等. 三穗鸭MSTN基因多态性与屠宰性状相关性研究[J].中国家禽,2013,35(23):11～15.
7 岳志刚. 绿头野鸭肌肉抑制素基因多态性及其与生产性能的关联性研究[D]. 北京:中国农业科学院,2010.
8 胡兰,郭东新,胡锐,等. 大骨鸡中MSTN基因表达规律性的研究[J]. 动物科学与动物医学,2003,20(11):24～27.
9 赵南南,陈永华,刘倩,等. 高邮鸭肌肉生长抑制素基因 (MSTN) 5'调控区多态性与生长及腹脂率的关联分析[J].畜牧与兽医,2013, 47(7):91～93.
10 戴晔. 北京鸭和樱桃谷鸭肉用性能及MSTN基因多态性的研究[D]. 杨凌:西北农林科技大学,2006.
11 Clop A, Mareq F, Takeda H, et al. A mutation creating a potential illegitimate microRNA target site in the myostatin gene affects muscularity in sheep[J]. Nature Genetics, 2006, 38:813～818.
12 Gay S,Jublanc E,Bonnieu A,et al.Myostatin deficiency is associated with an increase in number of total axons and motor axons innervating mouse tibialis anterior muscle[J].Muscle Nerve, 2012, 45(5):698～704.
13 Herpin A, Lelong C, Favrel P. Transforming growth factor-β-related protein,an ancestral and widespread superfamily of cytokines in metazoans [J]. Dev Comp Immunol,2004,28:461～485.
14 Lee S J, McPherron A C. Myostatin and the control of skeletal muscle mass[J]. Curr Opin Genet Dev,1999, 9(5):604～607.
15 Marchitelli C, Savarese M C, Crisa A, et al. Double muscling in Marchigiana beef breed is caused by a stop codon in the third exon of myostatin gene[J]. Mammalian Genome, 2003, 14:392～395.
16 McPherron A C, Lawler A M, Lee S J. Regulation of skeletal muscle mass in mice by a new TGF-β super family member[J]. Nature, 1997a, 387(6628):83～90.
17 McPherron A C, Lee S J. Double muscling in cattle due to mutations in the myostatin gene[J].Proc Natl Acad Sci, 1997b, 94(23):12457～12461.
18 Mosher D S, Quignon P, Bustamante C D, et al. A mutation in the myostatin gene increases muscle mass and enhances racing performance in heterozygous dogs[J]. PLoS Genetics, 2007,3(5):e79.
19 Schuelke M, Wagner K R, Stolz L E, et al.Myostatin mutation associated with gross muscle hypearophy in a child[J]. N Engl J Med, 2004, 351:1030～1031.
20 Thomas M, Langley B, Berry C, et al. Myostatin,a negative regulator of muscle growth functions by inhibiting myoblast proliferation[J]. Journal of Biological Chemistry, 2000, 275:40235～40243.

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Polymorphisms of MSTN Gene and its Relationship with Slaughter Traits in Xingyi Duck

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