microRNA-21和TGFBI基因在猪骨骼肌生长发育中的表达分析

Abstract

Abstract: The aim of this study was to investigate the correlativity between expression of microRNA-21 (miR-21) and transforming growth factor beta-induced (TGFBI) in skeletal muscles of Landrace pigs. Comparative analysis was conducted between miR-21 and TGFBI in different tissues and in skeletal muscles at 28 developmental stages by quantitative PCR (qPCR). The results showed that the miR-21 was expressed without specificity in different tissues and fluctuated in skeletal muscles at developmental stages; TGFBI was expressed highly in skeletal muscles at embryonic periods and has different expression levels during developmental periods of skeletal muscles. The correlativity analysis showed that there was significantly negative correlativity between miR-21 and TGFBI in embryonic period of skeletal muscle development, which indicated that TGFBI was regulated by miR-21 in pig skeletal muscle development.

Key words: miR-21; TGFBI; skeletal muscle; development; pig

CLC Number:

Q786

ZHU Shi-yun, XIE Bing-kun, LIANG Ru-yi, LI Kui, TANG Zhong-lin. Expression Analysis of microRNA-21 and TGFBI Genes in Skeletal Muscle of Pigs at Different Development Stages[J]. , 2014, 41(11): 191-197.

References

1 梁如意. microRNA-21对猪骨骼肌生长发育调控的研究[D].南京:南京农业大学,2011.
2 Asangani I A, Rasheed S, Nikolova D A, et al. microRNA-21 (miR-21) post-transcriptionally downregulates tumor suppressor Pdcd4 and stimulates invasion, intravasation and metastasis in colorectal cancer[J]. Oncogene, 2008, 27(15): 2128～2136.
3 Ashmore C R, Addis P B, Doerr L. Development of muscle fibers in the fetal pig[J]. J Anim Sci, 1973, 36(6): 1088～1093.
4 Bae J, Lee S, Kim J, et al. βig-h3 supports keratinocyte adhesion, migration, and proliferation through α3β1 integrin[J]. Biochem Bioph Res Co, 2002, 294(5): 940～948.
5 Betel D, Wilson M, Gabow A, et al. The microRNA. org resource: Targets and expression[J]. Nucleic Acids Res, 2008, 36(suppl 1): D149～D153.
6 Bushati N, Cohen S M. microRNA functions[J]. Annu Rev Cell Dev Biol, 2007, 23: 175～205.
7 Callis T E, Chen J, Wang D. microRNAs in skeletal and cardiac muscle development[J]. Dna Cell Biol, 2007, 26(4): 219～225.
8 Davoli R, Braglia S, Russo V, et al. Expression profiling of functional genes in prenatal skeletal muscle tissue in Duroc and Pietrain pigs[J]. J Anim Breed Genet, 2011, 128(1): 15～27.
9 Hayashi T, Koyama N, Azuma Y, et al. Mesenchymal miR-21 regulates branching morphogenesis in murine submandibular gland in vitro[J]. Dev Biol, 2011, 352(2): 299～307.
10 Hei T K, Zhao Y, Zhou H, et al. Mechanism of radiation carcinogenesis: Role of the TGFBI gene and the inflammatory signaling cascade[J]. Adv Exp Med Biol,2011,720: 163～170.
11 Hou X, Tang Z, Liu H, et al. Discovery of microRNAs associated with myogenesis by deep sequencing of serial developmental skeletal muscles in pigs[J]. PLoS One, 2012, 7(12): e52123.
12 Iorio M V, Ferracin M, Liu C, et al. microRNA gene expression deregulation in human breast cancer[J]. Cancer Res, 2005, 65(16): 7065～7070.
13 Kloosterman W P, Plasterk R H. The diverse functions of microRNAs in animal development and disease[J]. Dev Cell, 2006, 11(4): 441～450.
14 Liu C, Li B, Cheng Y, et al. MiR-21 plays an important role in radiation induced carcinogenesis in BALB/c mice by directly targeting the tumor suppressor gene βig-h3[J]. Int J Biol Sci, 2011, 7(3): 347～363.
15 Lu J, Getz G, Miska E A, et al. microRNA expression profiles classify human cancers[J]. Nature, 2005, 435(7043): 834～838.
16 Lytle J R, Yario T A, Steitz J A. Target mRNAs are repressed as efficiently by microRNA-binding sites in the 5' UTR as in the 3' UTR[J]. Proceedings of the National Academy of Sciences, 2007, 104(23): 9667～9672.
17 Mccarthy J J, Esser K A, Andrade F H. microRNA-206 is overexpressed in the diaphragm but not the hindlimb muscle of mdx mouse[J]. Am J Physiol Cell Physiol, 2007, 293(1): C451～C457.
18 Meng F, Henson R, Wehbe J H, et al. microRNA-21 regulates expression of the PTEN tumor suppressor gene in human hepatocellular cancer[J]. Gastroenterology, 2007, 133(2): 647～658.
19 Papagiannakopoulos T, Shapiro A, Kosik K S. microRNA-21 targets a network of key tumor-suppressive pathways in glioblastoma cells[J]. Cancer Res, 2008, 68(19): 8164～8172.
20 Potthoff M J, Olson E N. MEF2: A central regulator of diverse developmental programs[J]. Development, 2007, 134(23): 4131～4140.
21 Sarkar J, Gou D, Turaka P, et al. microRNA-21 plays a role in hypoxia-mediated pulmonary artery smooth muscle cell proliferation and migration[J]. Am J Physiol Lung C, 2010, 299(6): L861.
22 Sayed D, Hong C, Chen I, et al. microRNAs play an essential role in the development of cardiac hypertrophy[J]. Circ Res, 2007, 100(3): 416～424.
23 Shenouda S K, Alahari S K. microRNA function in cancer: Oncogene or a tumor suppressor[J]. Cancer Metast Rev, 2009, 28(3～4): 369～378.
24 Skonier J, Neubauer M, Madisen L, et al. cDNA cloning and sequence analysis of βig-h3, a novel gene induced in a human adenocarcinoma cell line after treatment with transforming growth factor-β[J]. Dna Cell Biol, 1992, 11(7): 511～522.
25 Tao J, Lu Q, Wu D, et al. microRNA-21 modulates cell proliferation and sensitivity to doxorubicin in bladder cancer cells[J]. Oncol Rep, 2011, 25(6): 1721～1729.
26 Wang J Y, Gao Y B, Zhang N, et al. Tongxinluo ameliorates renal structure and function by regulating miR-21-induced epithelial-mesenchymal transition in diabetic nephropathy[J]. American Journal of Physiology Renal Physiology, 2014,306(5):F486～F495.
27 Wigmore P M, Stickland N C. Muscle development in large and small pig fetuses[J]. J Anat, 1983, 137(Pt 2): 235.
28 Williams A H, Liu N, van Rooij E, et al. microRNA control of muscle development and disease[J]. Curr Opin Cell Biol, 2009, 21(3): 461～469.
29 Yang Y, Li Y, Liang R, et al. Dynamic expression of microRNA-127 during porcine prenatal and postnatal skeletal muscle development[J]. Journal of Integrative Agriculture, 2013,8(4): 1221～1234.
30 Zhang Z, Li Z, Gao C, et al. miR-21 plays a pivotal role in gastric cancer pathogenesis and progression[J]. Lab Invest, 2008, 88(12): 1358～1366.
31 Zhang J, Ying Z, Tang Z, et al. microRNA-148a promotes myogenic differentiation by targeting the ROCK1 gene[J]. J Biol Chem, 2012, 287(25): 21093～21101.
32 Zhao S, Zhang J, Hou X, et al. OLFML3 expression is decreased during prenatal muscle development and regulated by microRNA-155 in pigs[J]. International Journal of Biological Sciences, 2012, 8(4): 459～469.
33 Zhu S, Si M, Wu H, et al. microRNA-21 targets the tumor suppressor gene tropomyosin 1 (TPM1)[J]. J Biol Chem, 2007, 282(19): 14328～14336.

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Expression Analysis of microRNA-21 and TGFBI Genes in Skeletal Muscle of Pigs at Different Development Stages

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