饲料中沙丁胺醇酶联免疫前处理方法的探究

Abstract

Abstract: Objective of the study was to develop a preparation treatment for salbutamol in feed by enzyme-linked immunosorbent assay (ELISA).The assay optimized the influence factor of the preparation and used to extract salbutamol residues in feed.The results showed that the inhibition concentration (IC₅₀) for the developed ELISA was 0.606 ng/mL with the detection range of 0.221 to 1.658 ng/mL and R² was 0.9998.After optimization,pH 7.5,0.06 mol/L PBS with the dilution ratio of 10 was selected to extract salbutamol residues in feed and the limit of detection (LOD) was 5.0 ng/mL in feed.The recoveries ranged from 77% to 110% for the spiked samples (5 and 10 μg/kg),and RSD was less than 8%.

Key words: enzyme-linked immunosorbent assay (ELISA); salbutamol; preparation treatment; feed

CLC Number:

S816.17

LI Jing-ya, WANG Zhan-hui, WANG Wen-jun, JIANG Hai-yang, SHEN Jian-zhong. Study on Preparation Treatment for Salbutamol in Feed by Enzyme-linked Immunosorbent Assay[J]. , 2014, 41(11): 179-183.

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Study on Preparation Treatment for Salbutamol in Feed by Enzyme-linked Immunosorbent Assay

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