重组植物乳杆菌生物学特性比较研究

Abstract

Abstract: In order to further study the distribution and adhesion of Lactobacillus plantarum in animal gastrointestinal tract,biological characteristics of Lactobacillus plantarum (LP) and recombinant Lactobacillus plantarum with GFP marker gene (Lp-GFP) had been systematically compared.The results showed that the growth curve of Lp-GFP was basically the same as Lp and Lp-GFP growth speed slightly lagging behind.The optimum growth temperature of Lp and Lp-GFP were all 37 ℃ and the optimum initial pH were all 6.5.The high temperature resistance,tolerance to artificial gastric fluid and artificial intestinal fluid,tolerance to bile salt of Lp and Lp-GFP had the same tendency.There was no significant difference on anti-bacteria character of Lp and Lp-GFP (P>0.05).The sensitivity to drugs of Lp and Lp-GFP had no differences except erythromycin.In conclusion,recombination has no significant effect on biological characteristics of Lactobacillus plantarum.These results laid an important foundation for cultivation and mechanism study of reconstructed Lactobacillus plantarum.

Key words: Lactobacillus plantarum; green fluorescence protein; recombination; biological characteristics

CLC Number:

S816.3

WANG Jing, JI Hai-feng, WANG Si-xin, ZHANG Dong-yan, LIU Hui, WANG Ya-min. Comparative Study on Biological Characteristics of Recombinant Lactobacillus plantarum[J]. , 2014, 41(11): 144-149.

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Comparative Study on Biological Characteristics of Recombinant Lactobacillus plantarum

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