大肠杆菌噬菌体的分离及生物学特性研究

Abstract

Abstract: 4 of E.coli strains were isolated from the soil of chicken farm on the EMB medium, and further identified as Escherichia coli by 16S rDNA sequence analysis. One virulent phage ΦLY4 was isolated when propagated on the E.coli LY4 as host cells. The phage had the hexagonal head and tail after observing with negatively stained method by electron microscope. The drastic diameter of this phage was 2.5 to 3.8 mm in size, and its phage titer was about 2.0×10¹⁰ PFU/mL, the optimal multiplicity of infection was 0.0001. It kept high stability at the temperature of －20 to 37 ℃ and pH at 5.0 to 10.0. Its viability was lost completely at the simulated gastric conditions （pH 1.2）. The growth curve showed that its incubation and burst phase was 20 min, and the lysis ability was strong. All of these properties showed that the phage ΦLY4 had a potential application in the control of environmental pollution.

Key words: E.coli; bacteriophage; biological characteristic

LI Yang，WANG Jing，HUO Kai，LI Xiao-ying，KONG Jian，GU Wei2. Isolation and Characterization of Bacteriophage of E.coli Isolated from Soil of Chicken Farm[J]. .

References

1 王晓丽,赵树欣,赵璐,等.欧文氏菌噬菌体的分离纯化及其生物学特性研究［J］.工业微生物,2011,41(6):47～51.
2 王静,王劲,孙运峰,等.治疗性噬菌体制剂的研究进展［J］.食品与药品,2011,13(9): 366～370.
3 李陇平,张智英.金黄色葡萄球菌烈性噬菌体的分离鉴定和最佳保存方法研究［J］.中国畜牧兽医,2011,38(6): 141～146.
4 李灏,谢慧君,孔健,等.畜禽肠道致病菌噬菌体的生物学特性研究［J］.微生物学通报,2004,31(2): 10～13.
5 张利军,熊鸿燕,张耀,等.类炭疽芽孢杆菌裂性噬菌体的筛选及其杀菌效应的初步观察［J］.中国消毒学杂志,2005,22(1):5～9.
6 赵贵明,仉庆文,姚李四,等.阪崎肠杆菌噬菌体的分离及其生物学特性［J］.微生物学报,2008,48(10):1373～1377.
7 赵晨,王辂.噬菌体治疗——旧概念，新阶段［J］.微生物学通报,2011,38(11):1698～1704.
8 熊鸿燕.一种新型的微生物控制技术——噬菌体溶菌技术［J］.中国消毒学杂志,2006, 23(4): 342～344.
9 Barrow P, Lovell M,Berchieri A Jr. Use of lytic bacteriophage for control of experimental Escherichia coil septicemia and meniningitis in chickens and calves［J］. Clin Diagn Lab Immunol, 1998,5（3）:294～298.
10 Borysowski J, Górski A. Is phage therapy acceptable in the immunocompromised host［J］. International Journal of Infectious Diseases, 2008,12(5): 466～471.
11 Frece J, Kos B, Svetec I K, et al. Importance of S-layer proteins in probiotic activity of Lactobacillus acidophilus M92［J］. Applied and Environmental Microbiology, 2005, 982(2): 285～292.
12 Nagy E, Ivánovics G. Anthrax-specific“AP50-like” phage isolated from Bacillus cereus strains［J］. Acta Microbiol Acad Sci Hung, 1982, 29(2): 89～98.
13 Nicol C G, Denby L, Lopez-Franco O,et al. Use of in vivo phage display to engineer novel adenoviruses for targeted delivery to the cardiac vasculature［J］. FEBS Letters,2009,583(12): 2100～2107.
14 Pierce J C, Masker W E. A single-base change in gene 10 of bacteriophage T7 pem its growth on Shigella sonnei［J］. J Virol, 1988, 62(11): 4369.
15 Park S C, Shimamura I, Fukunaga M, et al. Isolation of bacteriophages specific to a fish pathogen, Pseudomonas plecoglossicida, as a candidate for disease control［J］. Applied and Environmental Microbiology,2000,4: 1416～1422.
16 Wang S, Kong J, Gao C, et al. Isolation of characterization of a novel virulent phage (pHiLdb) of Lactobacillus delbrueckii［J］. International Journal of Food Microbiology, 2010, 137(1): 22～27.

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Isolation and Characterization of Bacteriophage of E.coli Isolated from Soil of Chicken Farm

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