›› 2013, Vol. 40 ›› Issue (S1): 46-49.

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Determination on 6 Kinds of Corn Gibberellic Olefine Ketone Residues in the Cow Milk by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

HAN Juan-zhu1, TIAN Xiao-ling1, CONG Xin2, LI Xiang-zhen1, SU Wei-yi1, LI Yong-cai1, LI Xin-nan1   

  1. 1. Liaoning Quality Inspection and Test Center of Veterinary Feed and Animal Products, Shenyang 110016, China;
    2. Panjin Institute for Food and Grug Control, Panjin 124010, China
  • Received:2013-08-01 Online:2013-09-30 Published:2013-11-29

Abstract: Developing a method for determination on 6 kinds of corn gibberellic olefine ketone (α-zearalanol, β-zearalanol, α-zearalenol, β-zearalenol, zearalanone and zearalenone) in the milk. The milk was concentrated after extracted with acetonitrile and purified by immunoaffinity solid-phase extraction and then was determined on an ACQUITY UPLCTM BEH C18 column with gradient elution using methanol and water (containing 0.02% acetic acid) as mobile phases. The mass spectrometric acquisitions were carried out by means of multiple reaction monitoring (MRM) in electrospray negative ionization mode. The detection limits were 1.0 μg/L for 6 kinds of corn gibberellic olefine ketone. The calibration curves were linear from 2.5～100.0 μg/L. This method was specific, sensitive, and suitable for determination on 6 kinds of corn gibberellic olefine ketone in the milk.

Key words: corn gibberellic olefine ketone; ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS); cow milk

CLC Number: