分子鉴定2种猪食道口线虫熔解曲线分析试验的建立

Abstract

Abstract: Based on the IGS rDNA sequences of O.dentatum and O.quadrispinulatum, a pair of primers (MCF/MCR) was designed for developing a melting curve analysis assay for the identification and differentiation of two Oesophagostomum species. This assay allowed the specific identification and differentiation of O.dentatum and O.quadrispinulatum by melting curve analysis, with no amplicons being amplified from heterogenerous DNA samples. DNA sequencing confirmed the identity of the amplified products. The single egg of Oesophagostomum spp. and DNA extracted using What-man FTA card could also be detected by this assay. These findings showed that the melting curve analysis assay established was accurate, sensitive, specific and rapid, allowing the detection and identification of O.dentatum and O.quadrispinulatum from pigs.

Key words: Oesophagostomum dentatum; Oesophagostomum quadrispinulatum; melting curve analysis; molecular identification

CLC Number:

S852.72

SHU Li, CHENG Tian, WENG Ya-biao, LIN Rui-qing, ZOU Shang-shu. Establishment of Melting Curve Analysis Assay for Molecular Identification of Oesophagostomum spp. from Pigs[J]. , 2013, 40(9): 70-74.

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Establishment of Melting Curve Analysis Assay for Molecular Identification of Oesophagostomum spp. from Pigs

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