传染性法氏囊病病毒VP2基因在昆虫细胞中的表达

Abstract

Abstract: The VP2 gene of very virulent infectious bursal disease virus (vvIBDV) JM-1/10 strain was placed behind the CMV promoter of pcDNA-3.1(+).The CMV-VP2 gene was cloned into the baculovirus expression system pFastBac^TM Dual to build pFast-CMV-VP2.Transformed it to Escherichia coli DH10Bac.Recombinant baculovirus expression plasmid Bacmid-CMV-VP2 was screened.Bacmid-CMV-VP2 was used to be transfected into Sf9 insect cells to get the recombinant baculovirus vBac-CMV-VP2 and transduced into BHK-21 cells.After 48 to 72 hours,indirect immunofluorescence assay (IFA) was conducted to detect specific fluorescence.Western blotting analysis result showed target protein was expressed.The prepared recombinant vBac-CMV-VP2 could not only be expressed in insect cells, but also expressed in mammalian cells.

Key words: infectious bursal disease virus; VP2 gene; insect expression vector

CLC Number:

Q786

YANG Feng, LI Xian-wei, SUN Min-hua, YAN Fu-qiang, WEI Qing-lan, XIANG Bin, REN Tao. Expression of VP2 Gene of Infectious Bursal Disease Virus in Insect Cells[J]. , 2013, 40(9): 65-69.

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Expression of VP2 Gene of Infectious Bursal Disease Virus in Insect Cells

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