一株鸭源新城疫病毒毒力基因分析及其对鸭的致病性研究

Abstract

Abstract: To explore the epidemiological understanding of Newcastle disease virus (NDV) in ducks, a Newcastle disease virus (NDV-104) was isolated from duck in Guangdong province in 2009. The biologically characteristic, pathogenicity and F and HN gene were explored. The results showed that MDT, ICPI and IVPI of the isolated strain were 56.4 h, 1.95 and 1.64, respectively. Combined with the analysis of the amino acid sequence of the F protein cleavage site (112 to 117), indicating it belonged to velogenic NDVs. The pathogenicity results showed that the isolated strain was infectious and pathogenic in ducks. Genetic analysis revealed that the isolate strain belonged to the gene Ⅶd subtype. The homology analysis of F and HN protein's amino acid showed that the isolated strain shared 96.6% to 99.3% and 97.0% to 99.7%, respectively, with genotype Ⅶ NDVs since 2000. However, the F and HN protein's amino acid of the isolate strains were low similarity with the common vaccine strains, such as B1, V4, Clone30, Mukteswar and LaSota, and were 88.1% and 87.6% similarity with LaSota and V4, which were different from the classical strains of NDV.

Key words: duck; Newcastle disease virus; F gene; HN gene; pathogenicity

CLC Number:

S852.65

LI Xian-wei, SUN Min-hua, HE Jing, WANG Zhao-xiong, LIU Da-wei, YANG Feng, REN Tao. Analysis of the Virulent Genes of One Newcastle Disease Viruses Isolated from Duck and its Pathogenicity Experiment in Duck[J]. , 2013, 40(9): 27-32.

References

1 吴焕荣,刁有祥,李宏梅,等. 鸭源副黏病毒的致病性试验[J].中国兽医学报,2011,31(1):36~39.
2 辛朝安, 任涛, 罗开健,等. 疑似鹅副粘病毒感染诊断初报[J].养禽与禽病防治, 1997,16(1):5.
3 陆承平.最新动物病毒分类简介[J].中国病毒学,2005,20(6):682~688.
4 殷震,刘景华.动物病毒学[M].第2版.北京:科学出版社,1997.
5 袁远华,王俊峰,蔡丽丽,等. 一株基因Ⅸ型鸭源新城疫病毒F基因的克隆及序列分析[J].中国畜牧兽医,2012,39(6):61~64.
6 梁华丽,童富淡,华炯钢,等.一株分离的鹅副粘病毒的毒力、致病性和传播方式的研究[J].浙江农业学报,2007,19(3):151~155.
7 黄瑜,李文杨,程龙飞,等.番鸭副粘病毒1型的分离鉴定[J].中国预防兽医学报,2005,27(2):48~50.
8 Czegledi A, Ujvari D, Somogyi E,et al. Third genome size category of avian paramyxovirus serotype 1 (Newcastle disease virus) and evolutionary implications[J]. Virus Res, 2006, 120(1~2): 36~48.
9 Liu X F, Wan H P, Ni X X, et al. Pathotypical and genotypical characterization of strains of Newcastle disease isolated from outbreaks in chicken and goose flocks in some regions of China during 1985~2001[J]. Arch Virol, 2003, 148(7): 1387~1403.
10 OIE. Manual of diagnostic tests and vaccines for terrestrial animal: Mammals, birds and bees[M]. Paris, France: Biological Standards Commission, World Organisation for Animal Health, 2004.
11 Onapa O M, Christnsen H, Mukiibi G M, et al. A preliminary study of the role of ducks in the transmission of Newcastle disease virus to in-contact rural free-range chickens[J. Trop Anim Health Prod, 2006, 38: 285~289.
12 Qin Z M, Tan L T, Xu H Y, et al. Pathotypical characterization and molecular epidemiology of Newcastle disease viruse isolates from different hosts in china from 1996 to 2005[J]. Clinical Microbio, 2008, 46(2): 601~611.
13 Roy P, Venugopalan A T, Manvell R. Characterization of Newcastle disease viruses isolated from chickens and ducks in Tamilnadu, India[J]. Vet Res Conmun, 2000, 24(2): 135~142.
14 Vallat B, Allen G P. Manual of diagnostic tests and vaccines for terrestrial animals (mammals, birds and bee)[M]. 5th ed. Paris, France: World Organization for Animal Health (OIE), 2004.
15 Zhang R, Pu J, Su J L,et al. Phylogenetic characterization of Newcastle disease virus isolated in the mainland of China during 2001~2009[J]. Vet Microbio, 2010, 141: 246~257.

[1]	JIAO Linlin, CHENG Yuting, WU Qingguo, WU Shuang, WU Zhi, ZHU Shanyuan, QIAN Yingjuan. Prokaryotic Expression and Polyclonal Antibody Preparation of Duck Tembusu Virus Capsid Protein [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2395-2402.
[2]	YU Daxin, LIU Wenjing, LIU Dapeng, LIU Tong, ZHANG He, GAI Kai, LI Shunan, ZHANG Beibei, HOU Shuisheng, ZHOU Zhengkui. Genome-wide Association Studies on Mineral Elements in Breast Muscle of Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1287-1295.
[3]	TAO Qinghua, HUANG Caiyun, CHEN Chao, LIU Wei, SUN Ningning, LI Ang. Cloning and Tissue Expression Difference Analysis of LIF Gene in White Muscovy Duck [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1296-1306.
[4]	XIE Shouyu, WANG Ruimin, CUI Pengfei, SHI Kaichuang, WEI Xiankai, FENG Shuping, LONG Feng, QU Sujie, LU Wenjun, HUANG Jinshan, HUANG Fengmei, WEN Xinrui, YIN Yanwen, DENG Guohua. Molecular Characteristics of Complete Genomes of Two H3 Subtype Avian Influenza Virus Isolates Derived from Sea Duck in Guangxi Province [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1319-1328.
[5]	CHEN Jimin, LI Huihuang, LAI Lianjie, YANG Yinhua, YANG Yue, LIN Ruiyi. Advance on Detection Methods and Vaccines of Duck Tembusu Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1329-1339.
[6]	LIU Caixia, SHI Weidi, KOU Lijun, MA Xun, WANG Jing, GAO Shengjie, LYU Shuangfei, CHEN Xuke, ZENG Dongdong, KONG Cuilian, REN Huijie. Regulation of Transcription of Key Virulence Factors of Listeria monocytogenes by LIPI-4 EⅡC [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1340-1351.
[7]	WANG Lucai, SALANG Wenzhu, ZHANG Huanrong. Isolation,Identification and Drug Resistance and Pathogenicity Analysis of Salmonella Enteritidis from Avian [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1663-1674.
[8]	ZHANG He, LIU Dapeng, TANG Hehe, LIU Tong, YU Daxin, YU Simeng, GAI Kai, HOU Shuisheng, ZHOU Zhengkui. Identification and Analysis of the Lipidome of the Breast Muscle of Pekin Duck and Liancheng White Duck [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 904-912.
[9]	LIANG Mingqi, CHEN Wei, ZHANG Yanan, XIA Weiguang, HUANG Xuebing, LI Kaichao, ZHENG Chuntian, WANG Shuang. Effects of Biotin on Laying Performance,Egg Quality,Plasma Biochemical Parameters,Ovarian Development and Tibia Characteristics of Laying Ducks in Peak Laying Period [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 564-572.
[10]	LIU Zhenni, LEI Xiaowen, LI Jianjun, ZENG Qingyuan, TAN Donghai, ZHONG Yunping, ZHANG Qiang, XIE Hualiang. Effects of Fermented Navel Orange Pomace on Intestinal Morphology,Intestinal Mucosal Immune Function and Microbial Diversity of Muscovy Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 573-585.
[11]	LUO Gan, ZOU Hong, REN Shaoke, WU Chunxia, ZOU Yao, LOU Yinying, ZHOU Yang, CHENG Fangjun, GUO Jianhua. Detection of O-antigen,Virulence Gene and Drug Sensitivity Test of Pathogenic Escherichia coli from Duck [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 713-721.
[12]	ZHAO Luyuan, ZOU Chenhao, CAO Jiu'ai, GUO Yanshan, LIU Lijun, ZHAN Xiu'an, LIN Gang, LIU Bing, YU Dongyou. Effects of Low Level of Compound Organic Trace Minerals on Serum Biochemistry,Immune Function and Antioxidant Capacity of Meat Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 86-96.
[13]	ZHANG Wei, WU Hua, YIN Yajie, LI Songli, HOU Shaohua. Isolation and Identification of Novel Duck Reovirus and Its σC Gene Sequence Analysis [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3520-3529.
[14]	KANG Yuhui, ZHOU Hongshan, JIN Wenjie, QIN Aijian. Isolation, Identification and Pathogenicity of a Strain of Plesiomonas shigelloides from Geese [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3676-3682.
[15]	SHAO Mingzhu, ZHAO Yunqing, LI Songli, HOU Shaohua, ZHANG Yanying. Isolation and Identification of Escherichia coli from Dairy Cows in Hebei Province and Its Pathogenicity and Antibacterial Effect of Chinese Herbal Medicine in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3683-3691.

Analysis of the Virulent Genes of One Newcastle Disease Viruses Isolated from Duck and its Pathogenicity Experiment in Duck

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments