引发仔猪水样腹泻的多重耐药、高致病性大肠埃希菌的分离与鉴定

Abstract

Abstract: Piglets watery diarrhea with high pathogenity and high mortality rate happened in many farms in Changchun area, the diarrhea piglets repeatedly tried a variety of medications,but no effect came out. From the onset of piglets intestinal wall lining,we had separated and identified some drugresistant and highly pathogenic E.coli strains.After the biochemical test,and cloning and sequencing of 16S rRNA of the strains,we compared them with the sequences in GenBank,the isolated strains had 99% homology with the 16S rRNA of E.coli sequence. After animal testing, we determined that this kind of E.coli could cause piglets watery diarrhea. Drug sensitivity test results showed that drugs commonly used in E.coli disease as treatments such as gentamicin,ofloxacin and ciprofloxacin were invalid.

Key words: Escherichia coli; 16S rRNA; drug sensibility test; isolation and identification; watery diarrhea

CLC Number:

S852.61⁺4

XUE Yu-jia, YU Da-yong, CUI Min, LV Yan, YAN Guang-mou. Separation and Identification of Multi-drug Resistant and High Pathogenic Escherichia coli Causing Piglets Watery Diarrhea[J]. , 2013, 40(9): 204-208.

References

1 王健.仔猪腹泻常见病因及综合防制措施探讨[J].畜禽业,2010(1):8~10.
2 师福山,赵德明.禽大肠杆菌的分离与16S rRNA的鉴定[J]. 中国畜牧兽医,2009,36(2):111~113.
3 李永峰,任南琪,杨传平,等.16S rDNA 测序快速鉴定废水生物处理系统目标细菌[J].哈尔滨工程大学学报,2005,26(6): 806~810.
4 杨霞,陈陆,王川庆.16S rRNA基因序列分析技术在细菌分类中应用的研究进展[J]. 西北农林科技大学学报(自然科学版),2008,36(2):55~60.
5 罗雯,万雅各.采用16S rRNA 基因PCR 检测病原菌的研究进展[J].南昌大学学报(理科版),2000,24(3):302~306.
6 聂映,谭为,周磊,等.几例新型仔猪腹泻的诊治[J].中国畜牧兽医,2011,38(8):201~203.
7 郭海勇,吴静,许磊,等.16S rRNA基因序列在动物消化道细菌鉴定中的应用研究[J].安徽农业科学,2008,36(17):7152~7154.
8 Cee J E,Sacchi C T,Glass M B, et al. Use of 16S rRNA gene sequencing for rapid identification and differentiation of Burkhold eriapseudomallei and B.mallet[J]. J Clin Microbiol,2003,41(10):4647~4654.
9 Drancourt M,Berger P,Raoult D. Systematic 16S rRNA gene sequencing of a typical clinical isolates identified 27 new bacterial species associated with humans[J].J Clin Microbiol,2004,42(5):2197~2202.
10 Greisen K,Loeffelholz M,Purohit A,et al. PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid[J]. J Clin Microbiol,1994,32(2):335~351.
11 Li W,Raoult D,Fournier P E. Bacterial strain typing in the genomic era[J].FEMS Microbiol Rev,2009,33(5):892~916.
12 Mignard S, Flandrois J P. 16S rRNA sequencing in routine bacterial identification: A 30-month experiment[J].J Microbiol Methods,2006,67(3):574~581.
13 Woses C R. Bacterial evolution[J].Microbiol Rev,1987,51:221~271.

[1]	LU Yukui, LIU Jiaqi, ZHONG Jiacheng, CHEN Jidang, ZHU Wanjun, ZHANG Yishan, ZHANG Jipei. Isolation,Identification and Pathogenicity Analysis of Avibacterium paragallinarum from Geese [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2562-2572.
[2]	LI Zhijie, ZHAO Ying, MA Xin, WANG Meng, YU Sijiu, WANG Libin, PAN Yangyang. Research Progress on Isolation Methods and Main Reproductive Functions of Exosomes from Animal Follicular Fluid [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1480-1488.
[3]	TIAN Changqing, ZHANG Kunzhong, QI Yumei, SHI Wenjing, DONG Zhijie, ZHANG Haohao, HE Zengwen, ZHI Ji, ZHAO Xuehui, CHONG Qian, XUE Huiwen, GOU Huitian. Isolation,Identification and Biological Characteristics Analysis of a Strain of Listeria monocytogenes from Environment [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1543-1555.
[4]	CAO Yingying, LIANG Liang, ZENG Yirong, ZHONG Qinghua, YUAN Xiaofang, TANG Haibo, LENG Jing. Isolation,Identification and Biological Characteristics of Salmonella from Tupaia belangeri chinensis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1718-1728.
[5]	WANG Bingyi, MA Hongcai, ZOU Minghao, FAN Shijie, YUAN Zhenjie, Labaciren, DONG Hailong, ZENG Jiangyong. Isolation,Identification and Drug Resistance Analysis of Streptococcus from Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 745-753.
[6]	ZHONG Huachen, WANG Lifang, GUO Chenyang, LIU Jialin, SONG Jie. Isolation,Identification and Drug Resistance Analysis of Pathogenic Bacteria from Milk Samples and Environment of Mastitis in Inner Mongolia [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 817-826.
[7]	SUN Yu, ZHANG Hao, LI Changyang, TAO Yanzi, WANG Qiuju. Isolation,Identification and Biological Characteristics of Antioxidant Bacillus from Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 359-367.
[8]	WEI Jin, WANG Jia, ZHAO Haoran, LIU Bo, LIU Yuanjie, YAO Wensheng, LIU Yan, MA Xin. Isolation, Identification and Antimicrobial Susceptibility of Mycoplasma gallisepticum from Different Areas [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 368-376.
[9]	YANG Fumei, YANG Linfu, ZENG Cheng, YANG Zhi, WANG Kang, DUAN Gang, DAI Feiyan. Isolation,Identification and Biological Characterization Analysis of a Strain of Carnobacterium maltaromaticum from Barking Deer [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 398-407.
[10]	ZHANG Wei, WU Hua, YIN Yajie, LI Songli, HOU Shaohua. Isolation and Identification of Novel Duck Reovirus and Its σC Gene Sequence Analysis [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3520-3529.
[11]	XI Jing, YI Jihai, WANG Yueli, XU Zhenyu, LI Peidong, ZHANG Jiangwei, CHEN Chuangfu. Isolation, Identification and Genotype Analysis of Group A Bovine Rotavirus G10 Strain XJ-2022 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3530-3538.
[12]	LIU Xiaolan, LIU Changjin, YU Wenyang, LI Xiaoxiang, BIAN Yanchao, HUANG Xiaohua, LUO Feng, DENG Shunzhou. Isolation and Identification of Porcine Rotavirus Jiangxi Strain AY01 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3151-3162.
[13]	XU Minsheng, KE Haiyi, SHI Keda, YANG Dongxia, ZHAI Shaolun, ZANG Yingan, LI Chunling. Isolation and Identification of Actinobacillus pleuropneumoniae from Guangdong Province and Detection and Analysis of Drug Resistance Phenotype and Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3212-3225.
[14]	YANG Zemin, LI Shuang, JIN Zhengyu, LIAO Yixiao, YANG Ying, WEN Ming. Isolation,Identification and Biological Characteristics of a Strain of Lactobacillus plantarum from Carp [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2805-2811.
[15]	YIN Yajie, LIANG Ruiying, CUI Huan, MENG Lijia, NI Weiling, QIAO Sina, GUO Kangkang, ZHANG Bo, LI Songli, HOU Shaohua, DONG Shishan. Isolation,Identification of Duck Tembusu Virus,and Genetic and Evolutionary Analysis of Its Envelope Protein [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1497-1506.

Separation and Identification of Multi-drug Resistant and High Pathogenic Escherichia coli Causing Piglets Watery Diarrhea

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments