J亚群禽白血病病毒双抗体夹心ELISA检测方法的建立

Abstract

Abstract: A double-antibody sandwich ELISA (DAS-ELISA) was developed for the detection of subgroup J avian leukosis virus (ALV-J). Antibodies of DAS-ELISA were purified from the ALV-J gp85 mono-specific serum prepared by our laboratory earlier. Results of statistics analyses showed that DAS-ELISA had good specificity, repeatability and stability. The limitation of viral antigens for detection was 0.165 μg/mL. The coincidence between PCR and DAS-ELISA established here was 85.2%.

Key words: avian leukosis; subgroup J avian leukosis virus(ALV-J); double-antibody sandwich ELISA(DAS-ELISA)

CLC Number:

S854.4⁺3

LIAO Ya-lin, LIANG Yi-yu, WANG Xiu-long, FENG Min, TAN Li-qiang, CAO Wei-sheng. Development of a Double-antibody Sandwich ELISA for Detection of Subgroup J Avian Leukosis Virus[J]. , 2013, 40(9): 15-18.

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Development of a Double-antibody Sandwich ELISA for Detection of Subgroup J Avian Leukosis Virus

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