禽白血病病毒贵州流行株env基因克隆与序列分析

doi:10.16431/j.cnki.1671-7236.2019.09.023

Abstract

Abstract: In order to understand the genetic variation and molecular characteristics of avian leukaemia virus (ALV) endemic strains in Guizhou,this study designed and synthesized primers based on ALV env gene for the purpose of gene amplification,cloning and sequence analysis of avian leukaemia clinical cases.The results showed that 3 positive samples were selected from clinical cases.The target gene fragments with a size of 921 bp were obtained by PCR amplification and named as GZ-ALV-1,GZ-ALV-2 and GZ-ALV-3 strains.Sequence analysis results showed that the nucleotide homology of 3 ALV epidemic strains in Guizhou ranged from 97.2% to 97.6%,which was relatively high with ALV-J at home and abroad,ranging from 93.1% to 99.3%,while the homology with ALV subgroups A,B,C,D,E and K was only 51.4% to 53.2%.The phylogenetic tree analysis showed that 3 ALV epidemic strains in Guizhou were in the same branch as the reference strains of ALV-J subgroup,which further indicated that all the ALV strains detected in this paper were ALV-J subgroup,while in different branches compared with A,B,C,D,E and K subgroups.The gene mutation analysis showed that 37 same nucleotide mutations of 3 epidemic strains resulted in 17 amino acid mutation sites,including 9 variable points in the high-variable region of hr1 and hr2,and 1 variable point in the low-variable region of vr3.The results showed that 3 ALV strains in Guizhou were ALV-J subgroup,and env gene loci were mutated,and the variable loci were located in the high-variable region.The results provided basic data for the epidemic situation of avian leukemia and the prevention and control and purification of ALV in Guizhou province.

Key words: avian leukosis virus (ALV); env gene; cloning; sequence analysis

CLC Number:

S852.65⁺9.3

HE Ling, YANG Qiuming, YUAN Haiwen, YANG Yuan, WEN Guilan, CHENG Zhentao. Cloning and Sequence Analysis of env Gene of ALV Guizhou Strain[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(9): 2683-2690.

References

[1] WILLIAMS S M,REED W M,BACON L D,et al.Response of White leghorn chickens of various genetic lines to infection with avian leukosis virus subgroup J[J].Avian Diseases,2004,48(1):61-67.
[2] 胡晓田.苏皖部分地区禽白血病的流行病学调查和A亚群禽白血病病毒env基因的克隆表达[D].扬州:扬州大学,2014. HU X T.The epidemiological investigation of ALV infection in Jiangsu and Anhui provinces and expression of env gene of subgroup A of avian leukosis virus[D].Yangzhou:Yangzhou University,2014.(in Chinese)
[3] 黄新.贵州省地方品种鸡禽白血病病毒流行毒株的亚型鉴定及其分子特征分析[D].泰安:山东农业大学,2017. HUANG X.Subtype identification and molecular characteristics of avian leukosis virus prevalent strains of local chicken breeds in Guizhou province[D].Tai'an:Shandong Agricultural University,2017.(in Chinese)
[4] PAYNE L N,GILLESPIE A M,HOWES K.Induction of myeloid leukosis and other tumours with the HPRS-103 strain of ALV[J].Veterinary Record,1991,129(20):447-448.
[5] 徐晴晴,王峰,孙培,等.近年来ALV-J的国内外研究进展[J].中国兽医学报,2018,38(9):1820-1824. XU Q Q,WANG F,SUN P,et al.Research progress of ALV-J in recent years at home and abroad[J].Chinese Journal of Veterinary Medicine,2018,38(9):1820-1824.(in Chinese)
[6] PAYNE L N,GILLESPIE A M,HOWES K.Myeloid leukaemogenicity and transmission of the HPRS-103 strain of avian leukemis virus[J].Leukemia,1992,6(11):1167-1176.
[7] 杜岩,崔治中,秦爱建,等.从市场商品肉鸡中检出J亚群禽白血病病毒[J].中国家禽学报,1999,1(1):4. DU Y,CUI Z Z,QIN A J,et al.Detection of J subgroup avian leukosis virus from market commercial broilers[J]. Chinese Journal of Poultry,1999,1(1):4.(in Chinese)
[8] 何成伟,王佩坤,秦丽莉,等.广西凭祥斗鸡白血病病毒检测及分离株env基因分型[J].动物医学进展,2016,37(2):23-26. HE C W,WANG P K,QIN L L,et al.Detection of avian leukosis virus in game fowl of Pingxiang,Guangxi and env gene analysis of isolates[J].Progress in Veterinary Medicine,2016,37(2):23-26.(in Chinese)
[9] 崔治中.J亚群禽白血病病毒中国分离株的人工致病性试验[J].中国农业科学,2002,35(4):430-433. CUI Z Z.Study on pathogenicity of Chinese strains of subgroup J avian leukosis viruses[J].Scientia Agricultura Sinica,2002,35(4):430-433.(in Chinese)
[10] 崔治中.禽白血病及其防控百问百答(三)[J].中国家禽,2017,39(3):72-76. CUI Z Z.Avian leukemia and its prevention and control questions and answers (Ⅲ)[J].China Poultry,2017,39(3):72-76.(in Chinese)
[11] 林汉卿,陈绍品,温贵兰,等.禽白血病病毒贵州流行株gp85基因的序列分析[J].中国家禽,2017,39(12):25-29. LIN H Q,CHEN S P,WEN G L,et al.Sequence analysis of gp85 gene of avian leucosis virus epidemic strain in Guizhou[J].China Poultry,2017,39(12):25-29.(in Chinese)
[12] 钟兴福,王培坤,杨永立,等.广西地方禽类品种ALV和REV感染情况的调查及ALV分离株gp85基因序列分析[J].中国畜牧兽医,2015,42(7):1647-1653. ZHONG X F,WANG P K,YANG Y L,et al.Infection investigation of ALV and REV in Guangxi local poultry breeds and sequence analysis of gp85 gene of ALV isolates[J].China Animal Husbandry & Veterinary Medicine,2015,42(7):1647-1653.(in Chinese)
[13] 代阳,杨其峰,王波,等.不同地区海兰褐蛋鸡中J亚群——禽白血病病毒株gp85基因的分子演化分析[J].畜牧兽医学报,2010,41(5):635-638. DAI Y,YANG Q F,WANG B,et al.Molecular evolution analysis for gp85 gene of subgroup J-avian leukosis viruses strains from in Hyline egg-type chickens in different areas[J].Acta Veterinaia et Zootechnica Sinica,2010,41(5):635-638.(in Chinese)
[14] 杨骁,张训海.J亚型禽白血病研究进展[J].中国畜牧兽医,2014,41(6):230-235. YANG X,ZHANG X H.Research progress on subtype J avian leukosis[J].China Animal Husbandry & Veterinary Medicine,2014,41(6):230-235.(in Chinese)
[15] 杨波,高玉龙,高宏雷,等.我国东北地区野生鸟类A亚群禽白血病病毒分子流行病学调查及env基因序列分析[J].中国预防兽医学报,2013,35(3):245-247. YANG B,GAO Y L,GAO H L,et al.Molecular epidemiology and env gene sequence analysis of avian leukosis virus isolated from wild birds in China[J].Chinese Journal of Preventive Veterinary Medicine,2013,35(3):245-247.(in Chinese)
[16] 杨树青,王丽媛,贠鲁祥,等.贵州省地方品种鸡禽白血病病毒流行株的亚型鉴定及其分子特征分析[J].中国预防兽医学报,2017,39(3):233-236. YANG S Q,WANG L Y,YUN L X,et al.Subtype identification and molecular characterization of local breed chicken avian leukosis virus epidemic strains in Guizhou province[J].Chinese Journal of Preventive Veterinary Medicine,2017,39(3):233-236.(in Chinese)
[17] ZHOU D,XUE J,HE S,et al.Reticuloendotheliosis virus and avian leukosis virus subgroup J synergistically increase the accumulation of exosomal miRNAs[J].Retrovirology,2018,15(1):45.
[18] 马美哥,于蒙蒙,许传田,等.进口白羽肉种鸡J亚群禽白血病病毒的全基因组序列分析[J/OL].中国预防兽医学报,2018,网络首发. MA M G,YU M M,XU C T,et al.Epidemic identification of imported White feather broiler chicken leukemia[J/OL].Chinese Journal of Preventive Veterinary Medicine,2018,Epub ahead of print.(in Chinese)
[19] 刘功振,张洪海,刘青,等.感染J亚群禽白血病病毒的蛋鸡群中检测出B亚群禽白血病病毒[J].病毒学报,2009,6:445-451. LIU G Z,ZHANG H H,LIU Q,et al.Subgroup B avian leukosis virus was detected in laying hens infected with subgroup J avian leukosis virus[J].Chinese Journal of Virology,2009,6:445-451.(in Chinese)
[20] 刘绍琼,王波,张振杰,等.817肉杂鸡肉瘤组织分离出A、J亚型禽白血病病毒[J].畜牧兽医学报,2011,42(3):396-401. LIU S Q,WANG B,ZHANG Z J,et al.The separation of subgroup A and J ALV in soft tissue sarcomas of "817" broiler hybrids[J].Chinese Journal of Animal and Veterinary Sciences,2011,42(3):396-401.(in Chinese)
[21] 沈海玉,窦新红,许明.地方特色蛋鸡配套系亲本群中不同亚群禽白血病病毒的混合感染[J].中国家禽,2018,40(16):25-28. CHEN H Y,DOU X H,XU M.Mixed infection of different subgroups of avian leucosis virus in the parental group of local specialty laying hens[J].China Poultry,2018,40(16):25-28.(in Chinese)
[22] 刘公平,赵振芬,刘福安.PCR/RFLP鉴别禽白血病病毒[J].中国兽医学报,2001,3:243-245. LIU G P,ZHAO Z F,LIU F A.Identifiy of avian leukosis virus using polymerase chain reaction and restriction fragment length polymorphism (PCR/RFLP)[J].Chinese Journal of Veterinary Science,2001,3:243-245.(in Chinese)
[23] SMITH L M,BROWN S R,HOWES K,et al.Development and application of polymerase chain reaction (PCR) tests for the detection of subgroup J avian leukosis virus[J]. Virus Research,1998,54(1):87-98.
[24] 朱瑞豪.鸡三种免疫抑制病基因芯片检测方法的建立[D].新乡:河南科技学院,2015. ZHU R H.The distribution of gene chip for three kinds of avian immunosuppressive diseases[D].Xin-xiang:Henan Institute of Science and Technology,2015.(in Chinese)
[25] 韩雪清,林祥梅,侯义宏,等.禽流感病毒分型基因芯片的研制[J].微生物学报,2008,48(9):1241-1249. HAN X M,LIN X M,HOU Y H,et al.Oligonucleotide microarray for subtyping avian influenza virus[J].Journal of Microbiology,2008,48(9):1241-1249.(in Chinese)
[26] 戴银,赵瑞宏,胡晓苗,等.安徽省五华鸡J亚群禽白血病病毒gp85基因分子序列特征分析[J].中国畜牧兽医,2013,40(11):195-200. DAI Y,ZHAO R H,HU X M,et al.Sequence feature analysis of gp85 gene of avian leukosis virus subgroup J in Wuhua chickens in Anhui province[J].China Animal Husbandry & Veterinary Medicine,2013,40(11):195-200.(in Chinese)

Cloning and Sequence Analysis of env Gene of ALV Guizhou Strain

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	CHEN Meiting, ZHENG Congsen, LIANG Zexian, LIN Qiaoer, CHANG Chuanzhe, ZHOU Jun, FENG Jun, LI Linlin, QIN Limei. Cloning and Bioinformatics Analysis of env Gene of Avian Leukosis Virus Subgroup J Strain [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1785-1795.
[2]	FAN Bingfeng, LI Wen, LIU Lixiang, ZHAO Xiangyuan, SHAO Jing, LIN Lefeng, SUN Huimin, ZHANG Xulin, XU Baozeng. Cloning and Bioinformatics Analysis of AANAT Gene in Mink [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1307-1318.
[3]	LIU Hongrun, ZHU Siran, FENG Lingli, ZHANG Kun, YAN Gang, WANG Yubin, ZHANG Shuai, JIANG Shan, XU Di, LAN Ganqiu, LIANG Jing. Cloning,Bioinformatics Analysis and Tissue Expression Localization of CDO1 Gene in Large White Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1352-1363.
[4]	ZHANG Yongjie, SUI Zhiyuan, ZHANG Zhishuai, WANG Chenguang, LI Xiaojun, XING Feng. Cloning and Bioinformatics Analysis of NPTX1 Gene and Its Tissues Expression at Puberty in Dolang Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1048-1058.
[5]	WEI Yirong, ZHENG Zihua, ZHANG Sanbao, SONG Ying, JIANG Haiyu, SUN Wenyue, CHENG Pengfei, LIU Yufan, ZOU Jianwei, HUANG Yanna, PAN Yan, JIANG Qinyang. Cloning,Bioinformatics Analysis and Tissue Expression of UCP1 Gene in Nubian Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 440-450.
[6]	TANG Jingyu, DU Hanyu, MENG Chunchun, LIU Guangqing. Cloning,Bioinformatics Analysis and Subcellular Localization of Interferon-stimulated Gene 15 in Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2843-2854.
[7]	QI Nannan, QIAN Yonghang, LUO Gang, SITU Xuqi, LIU Jiying. Cloning, Bioinformatics Analysis and Tissue Expression Location of Porcine MORC2 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2431-2441.
[8]	ZHANG Hang, SHA Huiyang, LI Huawei, HUANG Liangzong, ZHAO Mengmeng. Cloning, Bioinformatics and Tissue Expression Analysis of TRIM3 Gene in Swine [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2474-2483.
[9]	YAN Yelian, ZHANG Mengya, LIU Qiuchen, WANG Xin, XU Changzhi, ZONG Yanfeng, ZHU Zhihua, WU Sucheng, SONG Yu, LI Yunsheng, ZHANG Yunhai, CAO Zubing. Research Progress of Epigenetic Reprogramming in Mammalian Somatic Cell Nuclear Transfer Embryo Development [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2259-2269.
[10]	WANG Baobao, JIN Hang, XIAN Yuhan, FENG Hongsheng, GAO Fengshan. Cloning SLA-1 Gene from Porcine Kidney Epithelial Cells and Analyzing its Molecular Structure Characteristics [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1671-1678.
[11]	ZHANG Jihu, MI Renisa, WANG Zixuan, LI Lin, XING Feng, LIU Shudong. Cloning and Bioinformatics of ERα Gene and Its Expression of Ovary Tissue in Cele Black Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1679-1687.
[12]	SHEN Xiang, CHEN Linghui, YAO Jingjie, WANG Sanhu, BAI Yueyu, ZHANG Xiaojian. Cloning,Bioinformatics and Expression Analysis of Bovine CCR7 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1244-1252.
[13]	ZHANG Zhishuai, SUI Zhiyuan, ZHANG Jihu, LI Qingjin, XING Feng. Cloning of CNP Gene and Its Tissue Expression Characteristics at Puberty in Dolang Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1402-1412.
[14]	SONG Chun, WANG Bolin, LI Mei, WEN Ming, WANG Kaigong, CHEN Changxiu, CHENG Zhentao. Isolation and Identification of Mycoplasma synoviae Guizhou Strain and Sequence Analysis of VlhA Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1516-1523.
[15]	HOU Jinxing, WANG Zhanhang, ZHU Junru, JIANG Yue, LIU Shujuan, AN Xiaopeng. Cloning, Bioinformatics and Tissue Expression Profile Analysis of CSN1S1 Gene in Guanzhong Dairy Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 857-865.