不同检测材料对ELISA检测禽白血病病毒-p27抗原结果的影响

doi:10.16431/j.cnki.1671-7236.2015.05.039

Abstract

Abstract: In order to study the effect of different test materials on the detected results of avian leukosis virus (ALV)-p27 antigen by ELISA, the cloacal swabs ALV-p27 antigen were detected by ELISA in 180-day local breeds hen, and the parts of the positive and negative chicken was selected to group test, the serum, egg whites and cell supernatant ALV-p27 antigen were detected by ELISA, the specific genes of ALV were detected by RT-PCR in blood.The results showed that serum, egg white and cell supernatant as ELISA test materials, the positive rate lower of than cloacal swabs, and serum ALV-p27 positive samples include all egg whites and cell supernatant positive samples in positive group.It was a significant correlation between ELISAs with serum and cell supernatant (linear equation:Y=1.8439X-0.1469, the correl was 0.937).In positive group, ALV-p27 gene positive rate lower than cloacal swabs ELISA, but higher than the serum, egg and cell culture medium, and ALV-p27 gene positive samples include serum positive samples by ELISA.ALV-J gp85 gene positive rate of 29.17%, and all positive samples were included in the serum ALV-p27 positive samples.The results suggested that the cloacal swabs as test material may occur false positive results, and egg whites and cell supernatant may occur undetected by ELISA ALV-p27 antigen assay in adult chicken, serum as test material in ELISA could more accurately reflect the state of adult chickens infected with ALV.

Key words: avian leukosis virus; ALV-p27 antigen; ELISA; test materials

CLC Number:

S858.31

SHEN Xue-huai, ZHANG Dan-jun, PAN Xiao-cheng, ZHAO Rui-hong, HU Xiao-miao, DAI Yin, HOU Hong-yan, ZHU Chuan-min. Effect of Different Test Materials on the Detected Results of Avian Leukosis Virus-p27 Antigen by ELISA[J]. , 2015, 42(5): 1294-1300.

References

[1] Shen Y, Cai L, Wang Y, et al.Genetic mutations of avian leukosis virus subgroup J strains extended their host range[J].J Gen Virol, 2014, 95(3):691-699.
[2] 乔彩霞, 关云涛, 陈洪岩.禽白血病研究进展[J].动物医学进展, 2003, 24(6):18-21.
[3] 崔治中, 张志, 杜岩.我国肉用型鸡群中J亚群白血病流行现状的调查[J].中国预防兽医学报, 2002, 24(4):292-294.
[4] 杨骁, 张训海.J亚型禽白血病研究进展[J].中国畜牧兽医, 2014, 41(6):230-235.
[5] 崔治中.鸡白血病及其鉴别诊断和预防控制[J].中国家禽, 2010, 32(8):1-12.
[6] 俞燕, 朱鸿媛, 高明燕, 等.地方鸡种禽白血病病毒感染动态及检测方法比较[J].中国家禽, 2014, 36(8):23-26.
[7] Crittenden L B, Smith E J.A comparision of test material for differentiating avian leukosis virus group-specific antigens of exogenous and endogenous origin[J].Avian Dis, 1984, 28(4):1057-1071.
[8] 奚德华, 梁有志, 尹丽萍, 等.规模化种禽场禽白血病检测方法的比较及初步应用[J].中国家禽, 2014, 36(3):14-17.
[9] 周刚, 牛成明, 司昌德, 等.内源和外源性禽白血病病毒鉴别PCR检测方法的建立[J].中国预防兽医学报, 2010, 32(7):546-549.
[10] 陈静, 程合刚, 王波, 等.PCR检测血液样品方法在禽白血病净化中的应用研究[J].中国家禽, 2014, 36(4):13-15.
[11] 王小芬, 刘雪美, 廖亚琳, 等.J亚型禽白血病病毒感染黄鸡病毒血症和抗体反应动态的研究[J].中国畜牧兽医, 2012, 39(8):174-178.
[12] 叶建强, 秦爱建.J亚群禽白血病病毒(ALV-J)ELISA 检测方法的建立[J].中国兽医学报, 2006, 26(3):235-237.
[13] 杭柏林, 胡建和, 李杰, 等.J亚群禽白血病病毒检测方法的研究进展[J].中国农学报, 2011, 27(32):21-24.
[14] Zeng X, Liu L, Hao R, et al.Detection and molecular characterization of J subgroup avian leukosis virus in wild ducks in China[J].PLoS One, 2014, 9(4):e94980.
[15] Qin L T, Gao Y L, Ni W, et al.Development and application of Real-time PCR for detection of subgroup J avian leukosis virus[J].Journal of Clinical Microbiology, 2013, 51(1):149-154.
[16] 崔治中.我国鸡群中禽白血病流行现状和对策[J].中国家禽, 2009, 31(13):1-3.
[17] 张小桃, 赖汉漳, 曹伟胜.禽白血病病毒检测方法研究进展[J].养禽与禽病防治, 2009, 4:4-6.
[18] 陈浩, 王一新, 赵鹏, 等.禽白血病/肉瘤病毒肿瘤基因及其与致肿瘤机制的关系[J].畜牧兽医学报, 2012, 43(3):336-342.
[19] Dong X, Ju S, Zhao P, et al.Synergetic effects of subgroup J avian leukosis virus and reticuloendotheliosis virus co-infection on growth retardation and immunosuppression in SPF chickens[J].Vet Microbiol, 2014, 172(3-4):425-431.
[20] 赵鹏, 崔治中, 马诚太.种蛋中禽白血病病毒p27抗原检出率与鸡群禽白血病发病率的相关性研究[J].畜牧兽医学报, 2012, 43(10):1618-1622.
[21] 王明月, 窦文文, 陈立坤, 等.不同组织样品或检测方法对ALV检测结果的影响[J].中国兽医学报, 2013, 33(5):659-663.
[22] 沈前程, 孙学高, 陈智武, 等.ALV抗原阳性公鸡精液人工授精对母鸡影响的试验报告[J].广西畜牧兽医, 2013, 29(6):346-348.
[23] 高娟, 姚俊峰, 王晓亮, 等.采用不同试剂盒检测禽白血病p27抗原阳性率的比较[A].中国畜牧兽医学会家禽学分会.中国畜牧兽医学会家禽学分会第九次代表会议暨第十六次全国家禽学术讨论会论文集[C].2013.
[24] Himl Y M, Foster D N, Bottoli I, et al.The DF-1 chicken fibroblast cell line:Transformation induced by diverse oncogenes and cell death resulting from infection by avian leukosis virus[J].Virology, 1998, 248:295-304.
[25] 陈瑞爱.鸡白血病生物防控措施要点[J].中国家禽, 2010, 32(7):36.
[26] 秦爱建.鸡白血病检测技术研究[J].中国家禽, 2010, 32(7):34-35.
[27] Witter R L, Bacon L D, Hunt H D, et al.Avian leukosis virus subgroup J infection profiles in broiler breeder chickens:Association with virus transmission to progeny[J].Avian Dis, 2000, 44(4):913-931.
[28] Pandiri A R, Mays J K, Silva R F, et al.Subgroup J avian leukosis virus neutralizing antibody escape variants contribute to viral persistence in meat-type chickens[J].Avian Dis, 2010, 54(2):848-856.
[29] Wang X Z, Wang B, Zhang P P, et al.The passage of cells can improve the detection rate of avian leukosis virus to facilitate the elimination of avian leukosis in chickens[J].Springerplus, 2013, 2(1):138.
[30] Saif Y M.苏敬良等译.禽病学[M].第12版.北京:中国农业出版社, 2011.
[31] 王秀臻, 张培培, 梁才芝, 等.净化鸡群中禽白血病所用检测材料的比较研究[A].中国畜牧兽医学会禽病学分会第十六次学术研讨会论文集[C].2012.
[32] 董宣, 刘娟, 赵鹏, 等.J亚群禽白血病病毒NX0101株的TCID₅₀与p27抗原之间的相关性研究[J].病毒学报, 2011, 27(6):521-525.
[33] Gopal S, Manoharan P, Kathaperumal K, et al.Differential detection of avian oncogenic viruses in poultry layer farms and turkeys by use of multiplex PCR[J].Journal of Clinical Microbiology, 2012, 50(8):2668-2673.
[34] Kiml Y B, Brown T P.Development of quantitative competitive-reverse transcriptase polymerase chain reaction for detection and quantitation of avian leukosis virus subgroup J[J].J Vet Diagn Invest, 2004, 16(3):191-196.

Effect of Different Test Materials on the Detected Results of Avian Leukosis Virus-p27 Antigen by ELISA

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHANG Fangyuan, LIN Yating, YANG Dawei, CHEN Hu, LI Guimei, SHAN Hu. Prokaryotic Expression of African Swine Fever Virus P30 Protein and Establishment of Indirect ELISA Detection Method [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2012-2022.
[2]	MU Yijiao, YU Ruiming, ZHANG Liping, WANG Yonglu. Establishment and Application of ELISA for Detection of IgA Antibody Based on the Recombinant Full-length S2 Protein of Porcine Epidemic Diarrhea Virus Variant Strain [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1185-1194.
[3]	NIU Xiaojie, XU Mingguo, XIAO Li, LIU Wenhao, CHEN Chuangfu, WANG Yong. Expression and Purification of IPAJ Protein of Salmonella Pullorum, Preparation of Polyclonal Antibody and Establishment of ELISA Method [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1218-1228.
[4]	ZHANG Kai, ZHANG Chunping, GE Shengqiang, SUN Chunxi, CHENG Jie, FU Chunyu, WANG Gang, NIU Xing, PENG Jun. Expression of p22 Protein of African Swine Fever Virus,Preparation of Polyclonal Antibody and Establishment of Indirect ELISA Method [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 270-279.
[5]	HAN Rui, YANG Xing, ZHANG Wenbo, CHEN Jialing, ZHOU Xiaoli. Prokaryotic Expression of Autolysin from Streptococcus suis and Establishment of an Indirect ELISA Method for Detection of Its Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3508-3519.
[6]	TANG Qingfeng, LI Ling, HUANG Jiaxiang, FANG Wenyuan, NONG Haoru, TANG Yan, LU Chengwei, WEI Panqiu, FENG Ling. Survey on Mycotoxin Contamination of Buffalo Milk in Different Seasons Under Different Feeding Modes in Guangxi [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3665-3675.
[7]	ZHANG Wenyan, WANG Yawen, YUAN Chen, FENG Yawen, TENG Zhaojian, SHANG Jialiang, LU Jicheng, SONG Qinye. Establishment of Indirect ELISA Method for Detection of African Swine Fever Virus Antibodies Based on Truncated p72 Protein [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2688-2697.
[8]	TIAN Guangyuan, WANG Yuchen, ZHOU Yaping, GUO Ting, ZHAO Hongmei, ZHAO Fengmiao, SUN Yajie, BIAN Yuchen, YU Jialiang, ZHOU Yuxia. Prokaryotic Expression and Indirect ELISA Method Establishment of P60_58aa-928aa Protein of Mycoplasma ovipneumoniae [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1960-1969.
[9]	WANG Leming, WANG Yurui, ZENG Zixuan, RAO Guoshun, WU Zhengjiao, JIN Weikun, WANG Dongying. Preparation of Cathepsin L1 Monoclonal Antibody Against Fasclala gigantica and Establishment of Double Antibody Sandwich ELISA [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 677-686.
[10]	CHEN Jing, WU Yan, LI Mei, YUE Jun, ZHU Erpeng, WEN Ming, ZHANG Shuangxiang, CHENG Zhentao. Prokaryotic Expression of LPPA Protein of Mycoplasma mycoides subsp. capri and Establishment of Indirect ELISA Method [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 318-327.
[11]	LI Dingwei, GAO Keli, ZENG Yang, FANG Chun, YANG Yuying, LIU Jing, LIANG Xiongyan. Construction of Lactic Acid Bacteria Expressing Avian Leukosis Virus Subgroup A Gp85 Protein [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3456-3463.
[12]	JIN Weikun, YUAN Xiangxiang, WANG Jinhui, HOU Linjing, ZHENG Mengwei, WU Zhengjiao, ZHANG Weiyu, DI Wenda. Establishment of Indirect ELISA for Bovine Fasciolasis Diagnosis Based on Chromatographic Component of Fasciola gigantica Excretory-secretory Products [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 3010-3018.
[13]	LI Xinping, CHEN Xiaojie, ZHAO Bing, LIU Qiangde, DING Zhanqiang, ZHANG Fei, TANG Huifen, CAO Jian, HOU Feng, LIU Yang, WANG Gang. Prokaryotic Expression of Clostridium chauvoei CctA Gene and Establishment of Its Indirect ELISA [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1876-1882.
[14]	LIU Manman, WEN Guilan, WEN Ming, ZHANG Xiaobo, HU Xuan, SUN Yun, ZHANG Xiuli. Diagnosis and Related Gene Analysis of Co-infection of Avian Leukemia Virus Subgroup J and Enterococcus faecalis [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4671-4680.
[15]	YUAN Xiaoqing, LI Lixia, TANG Tian, CHEN Shengnan, LIANG Xiaotong, HUANG Liangzong, SI Xingkui, ZHANG Haoji, SUN Xiutao, DUAN Wenxue, JIN Ningyi, LIU Hao. Prokaryotic Expression and Purification of rHis-G1_189aa-239aa Peptide of Batai Virus and Establishment of Indirect ELISA Method [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3770-3778.