env基因对J亚群禽白血病病毒体外感染和复制能力的影响

Abstract

Abstract: In order to rescue the env element-substitution virus, we constructed the recombinant plasmid pNX-HNenv that contained the whole myelomas strain ALV-J NX0101 genome, but with env element of hemangiomas strain ALV-J HN06. The plasmids were transfected into DF-1 cells and the rescued viruses were identified, respectively. The recombinant virus was stable in cell culture during nine passages, and could be identified by ALV-J specific monoclonal antibody JE9 and virus NX-HNenv was rescued. The results showed that the env substitution had no apparent influence on ALV-J virus rescue, virus entry and replication.

Key words: subgroup J avian leukosis virus; env gene; reversegenetics; replication

CLC Number:

Q344⁺.13

WU Xiao-chan, LI Jiao, CAO Wei-sheng, LIAO Ming. Effects of env Gene on Subgroup J Avian Leukosis Virus Infection and Replication[J]. , 2013, 40(9): 143-146.

References

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Effects of env Gene on Subgroup J Avian Leukosis Virus Infection and Replication

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