›› 2013, Vol. 40 ›› Issue (8): 83-88.

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Protective Effect of Sodium Selenite on Hepatocyte Apoptosis and the Decrease of Mitochondrion Transmembrane Potential Induced by Free Fatty Acids

ZHANG Peng-chun, CHEN Li-jun, FAN Rui-wei, GUO Miao-miao, GUO Zhi-hou, ZHANG Wei-qian, ZHANG Zhi-gang   

  1. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China
  • Revised:2013-05-01 Online:2013-08-20 Published:2013-08-16

Abstract: The objective of this study was to define the effect of sodium selenite on hepatocyte apoptosis and the decrease of mitochondrion transmembrane potential induced by free fatty acids (FFA). Primary rat hepatocytes were obtained with two-step collagenase perfusion method. We estimated hepatocyte apoptosis, liver function and mitochondrion transmembrane potential in FFA treated group with or without sodium selenite (0.1 μmol/L). Our data indicated that FFA changed dose-dependently hepatocyte apoptosis, liver function and mitochondrion transmembrane potential, whereas the presence of sodium selenite reversed these alternations.

Key words: sodium selenite; free fatty acids; hepatocyte apoptosis; liver function; mitochondrion transmembrane potential

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