蒙古羊骨髓间充质干细胞的分离培养及多向诱导分化

Abstract

Abstract: To explore the optimal methods of isolating, puring, proliferating of Mongolia sheep bone marrow mesenchymal stem cells (BMSC), and their multiple differentiation potentiality.Bone marrow (BM) was collected from one 30-day-old sheep by puncture method.BMSC were harvested by gradient centrifuge and adhesive culture methods.Growth curves and cells double time of passaged BMSC were determined.The P3 BMSC were cultured in vitro under adipogenic,osteogenic and chondrogenic inductive environments and induced into adipocytes,osteoblates,chrondrocytes.Their adipogenic,osteogenic and chondrogenic differentiation properties were confirmed by histological staining such as oil red,Alizarin Red, HE.The Mongolia sheep BMSC were uniform spindle-shaped in appearance,showed active proliferative capacity and had S shape of growth curve. They could be induced to differentiate into adipocytes,osteoblasts and chondrocytes under different induction medium. The Mongolia sheep BMSC showed strong proliferation and multiple differentiation potential.It proved that the cells isolated were indeed the bone marrow mesenchymal stem cells.

Key words: Mongolia sheep; bone marrow mesenchymal stem cells (BMSC); cells culture; multiple differentiation

CLC Number:

S826.8⁺2

WEI Lin-gai, LIU Zong-zheng, GAO Jin-fang, ZHOU Huan-min, ZHANG Yan-ru. Isolation, Culture and Multiple Differentiation Induction of Mongolia Sheep Bone Marrow Mesenchymal Stem Cell[J]. , 2013, 40(8): 7-12.

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Isolation, Culture and Multiple Differentiation Induction of Mongolia Sheep Bone Marrow Mesenchymal Stem Cell

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