猪Lbx2基因的克隆、序列分析及表达

Abstract

Abstract: In this study, the Lbx2 gene was amplified by PCR, then the gene mutation was identified by sequencing. The gene expression profile of different tissues in pig was analyzed by semi-quantitative RT-PCR method. The results showed that Lbx2 was lowly expressed in liver, kidney and spleen. A phylogenetic tree was constructed by aligning the amino acid sequences of different species, which revealed that the porcine Lbx2 had a closer genetic relationship with the Lbx2 of Bos taurus, Pongo abelii, Canis familiaris than with those of other species. Moreover, five mutations were detected in the Lbx2 genomic fragment, including one deletion mutation and four single nucleotide polymorphisms. These results would provide a foundation for further studies on Lbx2 function.

Key words: pig; Lbx2 gene; expression; bioinformatics analysis

CLC Number:

S828

CHAO Zhe, ZHENG Xin-li, SUN Rui-ping, WEI Li-min, LIU Quan-wei, LIU Hai-long, HUANG Li-li, WANG Feng. Cloning, Sequence Analysis and Expression of Lbx2 Gene in Pig[J]. , 2013, 40(8): 26-30.

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Cloning, Sequence Analysis and Expression of Lbx2 Gene in Pig

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