荷斯坦牛和西门塔尔牛冻精品质及其体外受精后早期胚胎发育的比较研究

Abstract

Abstract: The objectives of this study were to investigate differences in semen quality and development of embryos after in vitro fertilization with frozen-thawed sperm from either Holstein or Simmental bulls. Progressive motility, plasma membrane integrity and acrosome integrity rates of of frozen-thawed sperm from Holstein and Simmental bulls were assessed using visual observation, the hypoosmotic swelling test and Coomassie Blue staining method, and compared the cleavage and blastocyst rates after IVM/IVF with sperm of Holstein and Simmental bulls. The results showed that progressive motility (30.4% and 27.2%),plasma membrane integrity (41.96% and 36.22%) and acrosome integrity rates (77.02% and 73.02%) of frozen-thawed sperm from Holstein and Simmental bulls were all no significant differences (P>0.05), the cleavage (57.5% and 48.6%) and blastocyst rates (30.3% and 23.2%) after IVM/IVF with sperm of Holstein bulls resulted in significant improvement over sperm of Simmental bulls (P<0.05). It was concluded that there were significant differences on embryo developmental competence of in vitro fertilization using different bull semen.

Key words: Holstein bull; Simmental bull; semen quality; in vitro fertilization

CLC Number:

S823

LI Xiao-xia, WANG Hao, CAO Ping-hua, YU Xue-li, LI Ying-hua. Comparison on Sperm Quality and Embryos Development after in vitro Fertilization of Bovine Oocytes with Frozen-thawed Sperm from Holstein and Simmental Bulls[J]. , 2013, 40(6): 161-164.

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Comparison on Sperm Quality and Embryos Development after in vitro Fertilization of Bovine Oocytes with Frozen-thawed Sperm from Holstein and Simmental Bulls

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