鸭副黏病毒和鸭圆环病毒二重荧光定量PCR检测方法的建立

Abstract

Abstract: A duplex Real-time polymerase chain reaction(drRT-PCR) assay was developed and optimized to simultaneously detect duck paramyxovirus and duck circovirus in one reaction. Two sets of specific primers for duck paramyxovirus and duck circovirus, along with two TaqMan probes specific for each virus were used in the assay. This drRT-PCR assay was found to be specific and be able to detect and differentiate duck paramyxovirus and duck circovirus, and no positive results were observed, when nucleic acid from duck hepatitis virus, Muscovy duck parvovirus, duck plague virus and H9 subtype avian influenza virus and so on were used as drRT-PCR templates. The sensitivity of this drRT-PCR assay was 160 and 140 copies for duck paramyxovirus and duck circovirus,respectivly. The drRT-PCR assay was applied to 118 clinical samples detection. Among these samples, duck paramyxovirus positive rate was 0.85%, duck circovirus positive tate was 8.47%, no co-infection. This drRT-PCR assay was a quick, sensitive and specific test for detecting duck paramyxovirus and duck circovirus,and would be useful for the control of these viruses in ducks.

Key words: duck paramyxovirus; duck circovirus; duplex Real-time PCR

CLC Number:

XU Zong-li, XIE Zhi-xun, XIE Li-ji, LIU Jia-bo, XIE Zhi-qin, PANG Yao-shan, DENG Xian-wen, FAN Qing. Establishment of a Duplex Real-time PCR Assay for Detection of Duck Paramyxovirus and Duck Circovirus[J]. , 2013, 40(4): 26-31.

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Establishment of a Duplex Real-time PCR Assay for Detection of Duck Paramyxovirus and Duck Circovirus

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