一株鸭源禽Ⅰ型副黏病毒毒力测定及 诱导免疫应答研究

doi:10.16431/j.cnki.1671-7236.2017.04.032

Abstract

Abstract:

In this study,the biological and genetic characterization of a paramyxovirus type Ⅰ strain (GSFY strain) which was obtained from Muscovy duck were determined. Afterward,GSFY strain was developed as an inactivated oil emulsion vaccine. The protective evaluation of this new vaccine candidate was conducted on the Muscovy duck. The results showed that the mean death time (MDT) of chicken embryo,the intracerebral pathogenicity index (ICPI) of 1-day-old chicken and the intravenous pathogenicity index (IVPI) in 6-week-old chicken were 71 h,1.75 and 2.49, respectively. Homologous comparison of F gene nucleotide sequence showed that the GSFY strain shared 97.1% with Chicken/China/Guangxi 9/2003,which was the highest homology,and they all belonged to type Ⅶ. The results suggested that this newly isolated strain was the type Ⅶ high virulent paramyxovirus. The immunize protective evaluation results suggested that the mean antibody titer of 7 and 21 days after the third immunization were 6log2 and 8log2,respectively. When the immunized duck were attacked with GSFY strain,100% protective rate could be expected when the mean antibody titer was above 5log2. This study provided the basic data for vaccine development of avian type Ⅰ paramyxovirus.

Key words: duck paramyxovirus type Ⅰ; biological characterization; genetic characterization; inactivated oil emulsion vaccine

CLC Number:

S852.65

ZHANG Ji-pei, TAN Hua-long, CHEN Gang, ZENG Qiang, ZHANG Fu-ying, CHEN Jian-hong. Evaluation of Virulence and Immune Effect of a Duck Paramyxovirus Type Ⅰ Strain[J]. , 2017, 44(4): 1168-1174.

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Evaluation of Virulence and Immune Effect of a Duck Paramyxovirus Type Ⅰ Strain

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