China Animal Husbandry & Veterinary Medicine ›› 2013, Vol. 40 ›› Issue (4): 22-25.

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Preparation and Identification of Monoclonal Antibodies against Recombinant Truncated P Protein of Canine Distemper Virus

YUAN Wei-feng1, JIA Hong1, YU Ping-ping1,2, HOU Shao-hua1, GUO Xiao-yu1, SHI Li-jun1, ZHAO Zhan-zhong1, ZHU Hong-fei1   

  1. 1. Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China;
    2. College of Animal Science and Technology,Shandong Agriculture University,Tai’an 271017,China
  • Revised:2012-11-26 Online:2013-04-20 Published:2013-04-19

Abstract: The assay was aimed to prepare monoclonal antibodies (McAbs) against P1 protein of canine distemper virus (CDV). BALB/c mice were immunized with P1 protein and the immunized mice spleen myeloma cells were fused with SP2/0. Two hybridoma cell lines of E9E4C10 and E9F8D9 that stably produced antibodies against protein were identified by ELISA. The antibody titres of ascites for the hybridoma lines were both 1∶106. The subtypes of monoclonal antibodies were both identified as IgG2b. Western blotting and IFA showed that the McAbs specifically recognized certain antigenic epitopes of P1 protein of CDV. Additive index of ELISA competitive binding assay indicated that the two monoclonal antibodies were specific for different epitopes. The McAbs prepared in this study were useful tools for developing immunological diagnosis techniques of CDV and investing P protein function.

Key words: canine distemper virus; recombinant P protein; monoclonal antibodies; identification

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