Abstract: This assay was aimed to express the fusion protein of the MxA in Escherichia coli and prepare the antisera of rabbit against MxA.The recombinant expression vector pET32a(+)-MxA was contrusted by combinating MxA fragment from PCR and prokaryotic expression vector pET32a(+), recombinant protein was induced to express and be purified. We immuned New Zealand rabbits with purified recombinant protein to prepare MxA antisera. Then, ELISA results showed that antibody titer was 1∶64000,results of Western blotting showed that antisera had well combination with MxA.Results indicated that we had succesfully prepared immunogenic MxA protein and antisera which laid a foundation for further study on the methods of clinical diagnosis.

Key words: MxA gene; prokaryotic expression; antisera