弓形虫棒状蛋白17基因的克隆与序列分析

Abstract

Abstract: Toxoplasma gondii ROP17 gene was amplified from individual T.gondii strains from different geographical locations and hosts by PCR. These PCR products were subjected to be cloned and sequenced and ROP17 sequences were aligned using the ClustalX 1.81. The phylogenetic relationships among T.gondii strains were constructed using the software Mega 5.0 and Puzzle 5.2. The lengths of all ROP17 sequences were 1375 bp, and the A+T contents were 49.53% to 50.04%. The intra-specific variation among the T.gondii strains was up to 2.23%. The variation rate in amino acid sequences was 0 to 6.11%. Phylogenetic analysis revealed that ROP17 gene sequences could be used as a marker for studying genetic relationships of T.gondii isolates. The low variation in ROP17 gene sequences among T.gondii strains might indicate that ROP17 gene could be used as a potential anti-toxoplasmosis vaccine candidate molecule in further studies.

Key words: Toxoplasma gondii; ROP17 gene; clone; sequence analysis

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NIE Zi-xiong, ZHANG Nian-zhang, HU Qian-jiang, CHEN Rui-zhao, ZHOU Dong-hui, YUAN Zi-guo, LIN Rui-qing, ZHU Xing-quan. Cloning and Sequence Analysis of ROP17 Gene of Toxoplasma gondii[J]. , 2013, 40(4): 13-17.

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Cloning and Sequence Analysis of ROP17 Gene of Toxoplasma gondii

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