酵母CUP1基因的结构及其蛋白质鳌合金属离子功能的研究进展

Abstract

Abstract: Yeast (Saccharomyces cerevisiae) CUP1 encodes a cysteine-rich protein with 61 amino acids in length and 6165 u in molecular weight. Yeast CUP1 is a copper metallothionein responsible for resistance to copper. Yeast is induced by copper, and the CUP1 expression is greatly enhanced at the mRNA lever driven by the strong combination of the CUP1 promoter and the factor ACE1.This article summarizes the progress on the yeast CUP1 gene. It mainly describes the structure, the role of binding metal-ion, and the analysis on the promoter of CUP1 gene. Also, it describes the application of the CUP1 gene, especially for the CUP1 promoter, for the transgenic organisms. It plays an essential role on the further research of the physiological mechanism of CUP1 gene, and provides theoretical assistance for the transgenic organisms, especially for the transgenic animals and plants.

Key words: yeast; CUP1 gene; binding; promoter; transgene

CLC Number:

XIE Xiao-xian, WANG Qi-shan, CHEN Zhen-liang, MA Yu-fang, PAN Yu-chun. Research Progress on the Structure and the Function on Binding Metal-ion of Yeast CUP1 Gene[J]. China Animal Husbandry & Veterinary Medicine, 2013, 40(3): 62-67.

References

1 Bi W X,Kong F,Hu X Y,et al. Role of glutathione in detoxification of copper and cadmium by yeast cells having different abilities to express cup1 protein[J]. Toxicol Mech Method,2007,17(6):371～378.
2 Brenes-Pomales A,Lindegren G,Lindegren C C. Gene control of copper-sensitivity in Saccharomyces[J].Nature, 1955,176:841～842.
3 Butt T R, Sternberg E, Herd J, et al. Cloning and expression of a yeast copper metallothionein gene[J]. Gene,1984a,27(1):23～33.
4 Butt T R, Sternberg E J, Gorman J A, et al. Copper metallothionein of yeast, structure of the gene, and regulation of expression[J]. Proc Natl Acad Sci,1984b,81(11):3332～3336.
5 Butt T R, Ecker D J. Yeast metallothionein and applications in biotechnology[J]. Microbiol Mol Biol R,1987,51(3):351～364.
6 Culotta V C,Hsu T,Hu S,et al. Copper and the ACE1 regulatory protein reversibly induce yeast metallothionein gene transcription in a mouse extract[J]. Proc Natl Acad Sci,1989,86(21):8377～8381.
7 Culotta V,Howard W,Liu X.CRS5 encodes a metallothionein-like protein in Saccharomyces cerevisiae[J]. J Biol Chem,1994,269:25295～25302.
8 Cusick K D,Boyer G L,Wilhelm S W,et al. Transcriptional profiling of Saccharomyces cerevisiae upon exposure to saxitoxin[J]. Environ Sci Technol,2009,43(15):6039～6045.
9 Fogel S,Welch J W. Tandem gene amplification mediates copper resistance in yeast[J]. Proc Natl Acad Sci,1982,79(17):5342～5346.
10 Fogel S,Welch J W,Cathala G. Gene amplification in yeast: CUP1 copy number regulates copper resistance[J]. Curr Genet,1983,7(5):347～355.
11 Fujita V S, Thiele D J, Coon M J. Expression of alcohol-inducible rabbit liver cytochrome P-450 3a (P-450ⅡE1) in Saccharomyces cerevisiae with the copper-inducible CUP1 promoter[J]. DNA Cell Biol,1990,9(2):111～118.
12 Furst P, Hu S H R, Hamer D. Copper activates metallothionein gene transcription by altering the conformation of a specific DNA binding protein[J]. Cell,1988,55:705～717.
13 Gaykalova D A, Nagarajavel V, Bondarenko V A,et al. A polar barrier to transcription can be circumvented by remodeler-induced nucleosome translocation[J]. Nucleic Acids Res,2011,39(9):3520～3528.
14 Gomes D S, Riger C J, Pinto M L, et al. Evaluation of the role of Ace1 and Yap1 in cadmium absorption using the eukaryotic cell model Saccharomyces cerevisiae[J]. Environ Toxicol Phar,2005,20(3):383～389.
15 Hasegawa I, Terada E, Sunairi M, et al. Genetic improvement of heavy metal tolerance in plants by transfer of the yeast metallothionein gene (CUP1)[J]. Plant Soil,1997,196(2):277～281.
16 Hawthorne D, Mortimer R. Chromosome mapping in Saccharomyces: Gentromere-linked genes[J]. Genetics,1960,45(8):1085～1110.
17 Hirasawa T, Nakakura Y, Yoshikawa K, et al. Comparative analysis of transcriptional responses to saline stress in the laboratory and brewing strains of Saccharomyces cerevisiae with DNA microarray[J]. Appl Microbiol Biot,2006,70(3):346～357.
18 Jensen L T, Howard W R, Strain J J, et al. Enhanced effectiveness of copper ion buffering by CUP1 metallothionein compared with CRS5 metallothionein in Saccharomyces cerevisiae[J]. J Biol Chem,1996,271(31):18514～18519.
19 Kägi J H R. Overview of metallothionein[J]. Method Enzymol,1991,205:613～626.
20 Karin M, Najarian R, Haslinger A, et al. Primary structure and transcription of an amplified genetic locus: The CUP1 locus of yeast[J]. Proc Natl Acad Sci,1984,81(2):337～341.
21 Karpova T S, Kim M J, Spriet C, et al. Concurrent fast and slow cycling of a transcriptional activator at an endogenous promoter[J]. Science,2008,319(5862):466～469.
22 Knudsen C B, Bjrnsdottir I, Jns O, et al. Detection of metallothionein isoforms from three different species using on-line capillary electrophoresis-mass spectrometry[J]. Anal Biochem,1998,265(1):167～175.
23 Krizkova S,Zitka O,Adam V,et al. Possibilities of electrochemical techniques in metallothionein and lead detection in fish tissues[J]. Czech J Anim Sci,2007,52:143～148.
24 Kuo H C,Moore J D,Krebs J E. Histone H2A and Spt10 cooperate to regulate induction and autoregulation of the CUP1 metallothionein[J]. J Biol Chem,2005,280(1):1004～1011.
25 Leblanc B P,Benham C J,Clark D J.An initiation element in the yeast CUP1 promoter is recognized by RNA polymerase Ⅱ in the absence of TATA box-binding protein if the DNA is negatively supercoiled[J]. Proc Natl Acad Sci,2000,97(20):10745～10750.
26 Li L,Kaplan J. A mitochondrial-vacuolar signaling pathway in yeast that affects iron and copper metabolism[J]. J Biol Chem,2004,279(32):33653～33661.
27 Liu X D,Thiele D J.Yeast metallothionein gene expression in response to metals and oxidative stress[J]. Methods, 1997,11(3):289～299.
28 Macek T, Mackova M, Pavlíkova D, et al. Accumulation of cadmium by transgenic tobacco[J]. Acta Biotechnol,2002,22:101～106.
29 Macreadie I G, Horaitis O, Vaughan P R,et al. Constitutive expression of the Saccharomyces cerevisiae CUP1 gene in Kluyveromyces lactis[J]. Yeast,1991,7(2):127～135.
30 Mascorro-Gallardo J, Covarrubias A, Gaxiola R. Construction of a CUP1 promoter-based vector to modulate gene expression in Saccharomyces cerevisiae[J]. Gene,1996,172(1):169～170.
31 McCormick P M, Cannon G C, Heinhorst S. Expression of the copper metallothionein CUP1 from Saccharomyces cerevisiae in the cyanobacterium synechococcus R2-Pim8 (Smta)[J]. Curr Microbiol,1999,38(3):155～162.
32 Meyer J L, Hoy M A, Jeyaprakash A. Insertion of a yeast metallothionein gene into the model insect drosophila melanogaster (Diptera: Drosophilidae) to assess the potential for its use in genetic improvement programs with natural enemies[J]. Biol Control,2006,36(2):129～138.
33 Niazi J H,Sang B I,Kim Y S,et al. Global gene response in Saccharomyces cerevisiae exposed to silver nanoparticles[J]. Appl Biochem Biotech,2011,164(8):1278～1291.
34 Pagani A,Villarreal L,Capdevila M, et al. The Saccharomyces cerevisiae CRS5 metallothionein metal-binding abilities and its role in the response to zinc overload[J]. Mol Microbiol,2007,63(1):256～269.
35 Palacios O, Atrian S, Capdevila M. Zn- and Cu-thioneins: A functional classification for metallothioneins?[J]. J Biol Inorg Chem,2011,16(7):991～1009.
36 Pavlikova D, Macek T, Mackova M, et al. The evaluation of cadmium, zinc and nickel accumulation ability of transgenic tobacco bearing different transgenes[J]. Plant Soil Environ,2004,50(12):513～517.
37 Premakumar R, Winge D, Wiley R, et al. Copper-chelatin:Isolation from various eucaryotic sources[J]. Arch Biochem Biophys,1975,170:278～288.
38 Rutherford J C, Bird A J. Metal-responsive transcription factors that regulate iron, zinc, and copper homeostasis in eukaryotic cells[J]. Eukaryot Cell,2004,3(1):1～13.
39 Shobayashi M, Ukena E, Fujii T, et al. Genome-wide expression profile of sake brewing yeast under shaking and static conditions[J]. Biosci Biotec Bioch,2007,71(2):323～335.
40 Silar P, Butler G, Thiele D. Heat shock transcription factor activates expression of a the yeast metallothionein gene[J]. Mol Cell Biol,1991,11:1232～1238.
41 Sorger P K, Pelham H R B. Yeast heat shock factor is an essential DNA-binding protein that exhibits temperature-dependent phosphorylation[J]. Cell,1988,54(6):855～864.
42 Steinle A,Oppermann-Sanio F B,Reichelt R,et al. Synthesis and accumulation of cyanophycin in transgenic strains of Saccharomyces cerevisiae[J]. Appl Environ Microbiol,2008,74(11):3410～3418.
43 Stroobants A, Delroisse J M, Delvigne F, et al. Isolation and biomass production of a Saccharomyces cerevisiae strain binding copper and zinc ions[J]. Appl Biochem Biotechnol,2009,157(1):85～97.
44 Tamai K, Liu X, Silar P, et al. Heat shock transcription factor activates yeast metallothionein gene expression in response to heat and glucose starvation via distinct signaling pathways[J]. Mol Cell Biol,1994,14(12):8155～8165.
45 Tamai K T, Gralla E B, Ellerby L M, et al. Yeast and mammalian metallothioneins functionally substitute for yeast copper-zinc superoxide dismutase[J]. Proc Natl Acad Sci,1993,90(17):8013～8017.
46 Thiele D J. Metal-regulated transcription in eukaryotes[J]. Nucleic Acids Res,1992,20(6):1183～1191.
47 Thomas J C, Davies E C, Malick F K, et al. Yeast metallothionein in transgenic tobacco promotes copper uptake from contaminated soils[J]. Biotechnol Prog,2003,19(2):273～280.
48 Wang D, Wang Z, Liu N, et al. Genetic modification of industrial yeast strains to obtain controllable NewFlo flocculation property and lower diacetyl production[J]. Biotechnol Lett,2008,30(11):2013～2018.
49 Wang Z Y, Wang J J, Liu X F, et al. Recombinant industrial brewing yeast strains with ADH2 interruption using self-cloning GSH1+ CUP1 cassette[J]. FEMS Yeast Res,2009,9(4):574～581.
50 Wegner S V, Sun F, Hernandez N, et al. The tightly regulated copper window in yeast[J]. Chem Commun,2011,47(9):2571～2573.
51 Weser U, Hartmann H J, Fretzdorff A, et al. Homologous copper(Ⅰ)-(thiolate)2-chromophores in yeast copper thionein[J]. Biochim Biophys Acta,1977,493(2):465～477.
52 Winge D R, Jensen L T, Srinivasan C. Metal-ion regulation of gene expression in yeast[J]. Curr Opin Chem Biol,1998,2(2):216～221.
53 Winge D R. Copper metalloregulation of gene expression[J]. Adv Protein Chem,2002,60:51～92.
54 Winge D R, Nielson K B, Gray W R, et al. Yeast metallothionein sequence and metal-binding properties[J].J Biol Chem,1985,260(27):14464～14470.
55 Wostowski T. Involvement of metallothionein and copper in cell proliferation[J]. Biometals,1993,6:71～76.
56 Xu J, Tian Y S, Peng R H, et al. Yeast copper-dependent transcription factor ACE1 enhanced copper stress tolerance in Arabidopsis[J]. BMB Rep,2009,42(11):752～757.
57 Zakalskiy A E, Zakalska O M, Rzhepetskyy Y A, et al. Overexpression of (His)₆-tagged human arginaseⅠin Saccharomyces cerevisiae and enzyme purification using metal affinity chromatography[J]. Protein Expres Purif,2012,81(1):63～68.
58 Zhang J G, Liu X Y, He X P, et al. Improvement of acetic acid tolerance and fermentation performance of Saccharomyces cerevisiae by disruption of the FPS1 aquaglyceroporin gene[J]. Biotechnol Lett,2011,32(2):277～284.
59 Zhou P, Thiele D. Rapid transcriptional autoregulation of a yeast metalloregulatory transcription factor is essential for high-level copper detoxification[J].Gene Dev,1993,7(9):1824～1835.

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Research Progress on the Structure and the Function on Binding Metal-ion of Yeast CUP1 Gene

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