›› 2013, Vol. 40 ›› Issue (3): 59-61.

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The PCR Detection and Gene Analysis of Torque-Teno Canis Virus in Different Extraction Samples

YU Da-yi1, YE Jian-bo2, YUAN Cong-li2, HUA Xiu-guo2   

  1. 1. Animal Disease Control Center of Minhang District, Shanghai 201109, China;
    2. Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, ShanghaiJiao tong University, Shanghai 200240, China
  • Received:2012-08-29 Online:2013-03-20 Published:2013-03-19

Abstract: Based on the sequence of Torque-Teno canis virus registered on NCBI (Cf-TTV10, accession No.AB076002), we designed primers for nested PCR to detect the Torque-Teno canis virus in serum and fecal samples. It was showed that there were 14.6% Torque-Teno canis virus positive canine serum samples and 12.7% positive fecal samples, and it was verified that the gene type was same. There was no significant difference in different extraction samples(P>0.05). This research provided the basis for the further study of TTV characteristics and molecular epidemiology.

Key words: Torque-Teno canis virus; PCR detection; gene analysis

CLC Number: