猪瘟病毒E2蛋白主要抗原区编码基因的原核表达及抗原性研究

Abstract

Abstract: This study was aimed to analyze antigenicity of E2 protein of classical swine fever virus (CSFV). The E2 was amplified by RT-PCR from the genomic RNA of CSFV C-strain and cloned into expression vector pET-30a-c(+) to obtain recombinant pET30a-E2. The truncated E2 protein was expressed with high level in transformed Rosetta (DE3) after induction with IPTG. The results of SDS-PAGE and Western blotting indicated that the gene cloned in downstream of pET30a-E2 was expressed in high level and the recombinant fusion protein had immunologically reactive.

Key words: classical swine fever virus; E2 protein; prokaryotic expression; antigenicity

CLC Number:

S852.65

LIU Ping, WANG Hong-bo, QI Guang-yu, LIU Xue-rong, MU Ke-bin. The Prokaryctic Expression of the Main Antigenic Domains of E2 Protein of Classical Swine Fever Virus and Study on its Antigenicity[J]. , 2013, 40(3): 43-45.

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The Prokaryctic Expression of the Main Antigenic Domains of E2 Protein of Classical Swine Fever Virus and Study on its Antigenicity

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