日粮蛋白质水平对乌金猪肝脏组织脂类代谢相关基因表达的影响

Abstract

Abstract: The purpose of this study was to investigate the effect of dietary protein levels on the expression of hepatic lipid metabolism related genes in Wujin pig livers. Fifty-four Wujin pigs with the weight of 15 kg were randomly divided into 3 groups and 18 pigs per group,and given to three different protein levels with low dietary protein (14%,12% and 10%),medium dietary protein (16%,14% and 12%) and high dietary protein (18%,16% and 14%) during the growth stage of 15 to 30,30 to 60 and 60 to 100 kg body weight. At the body weight of 30,60 and 100 kg,pigs were slaughtered and liver samples were taken for analyzing the expression levels of lipid metabolism genes by Real-time PCR. The results showed that hepatic expression of acetyl-CoA carboxylase (ACC),fatty acid synthase (FAS) and sterol regulatory element binding protein 1c (SREBP-1c) reduced while the expression of carnitine palmitoyltransferase Ⅰ (CPT-Ⅰ) and peroxisome proliferator-activated receptorα (PPARα) increased as the protein content of diets increased. The study indicated that elevated level of dietary protein significantly decreased the expression of SREBP-1C,ACC,FAS (P<0.05),but increased the expression of CPT-Ⅰ,PPARα (P<0.05). Thus,the reduced synthesis and the increased oxidation of fatty acid collectively decreased the TG level transported to animal tissues and reduced the fat deposition in the body.

Key words: Wujin pigs; protein levels; lipid metabolism; Real-time PCR

CLC Number:

S858.28

YANG Ming-hua, LI Yong-neng, HUANG Ying, PAN Hong-bin, ZHAO Su-mei, GAO Shi-zheng. Effect of Dietry Protein Levels on the Expression of Hepatic Lipid Metabolism Related Genes in Wujin Pigs[J]. , 2013, 40(2): 108-112.

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Effect of Dietry Protein Levels on the Expression of Hepatic Lipid Metabolism Related Genes in Wujin Pigs

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