Abstract: The experiment was used enzyme immunoassay technique to develop a medroxyprogesterone kit which could rapidly tested in chicken. After testing, the limit of detection was 1 μg/kg in chicken samples,and the intra- and inter-relative standard deviations were less than 10%. The stability test results revealed that the kit could be stored for 12 months at 4℃. The cross-reactive assay showed that the specificity of monoclonal antibody was good. The kit operation time only needed 45 min,which could be suitable for detection of a large number of samples tested rapidly on the spot.

Key words: medroxyprogesterone; enzyme linked immunosorbent assay kit; chicken; rapid detection