鸭新城疫病毒与番鸭细小病毒二重PCR方法的建立

Abstract

Abstract: According to the gene sequences of duck Newcastle disease virus (NDV) L gene and Muscovy duck parvovirus (MDPV) Vp3 gene in GenBank, two sets of specific primers were designed by Primer Premier 5.0. The duplex PCR assay was developed through optimization of reaction conditions and validation of specificity, sensitivity and repetitiveness of the method. The sensitivity of the assay was 30 fg for NDV and 16 fg for MDPV. No specific bands of the same sizes were amplified from other duck pathogens, such as duck plague virus, duck hepatitis virus, duck circovirus, H9 subtype avian influenza virus, duck flavivirus, Salmonella, E.coli, avian Pasteurella multocida. This duplex PCR assay was a quick, sensitive and specific test for detection of duck NDV and MDPV, and would be useful for the control of these viruses in ducks.

Key words: duck Newcastle disease virus; Muscovy duck parvovirus; duplex PCR

CLC Number:

ZHANG Yan-fang, XIE Zhi-xun, XIE Li-ji, LIU Jia-bo, FAN Qing, PANG Yao-shan, DENG Xian-wen, XIE Zhi-qin. Establishment of a Duplex PCR Assay for Detection of Duck Newcastle Disease Virus and Muscovy Duck Parvovirus[J]. , 2013, 40(11): 63-66.

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Establishment of a Duplex PCR Assay for Detection of Duck Newcastle Disease Virus and Muscovy Duck Parvovirus

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