H9亚型禽流感病毒感染引起MDCK细胞内抗氧化功能的变化

Abstract

Abstract: After MDCK cells were seeded in 6-well culture plate at a density of 8×10⁴/cm²,avian influenza virus subtype H9 were inoculated with those cells at different doses (0,10^-3×EID₅₀,10^-4×EID₅₀and 10^-5×EID₅₀).Experimental results showed that compared with the control group, hydrogen peroxide (H₂O₂) and hydroxyl radical (·OH) in MDCK cells inoculated high-dose of avian influenza virus subtype H9 enhanced significantly (P>0.05),and superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity significantly decreased (P>0.05) in those cells. In addition,intracellular malondialdehyde (MDA) had a significant increasing (P>0.05) in MDCK cells treated with high-dose of virus. In conclusion,avian influenza virus subtype H9 could significantly enhance the content of reactive oxygen species (ROS) in MDCK cells, reduce the activities of antioxidant enzymes,and hinder ROS scavenging mechanisms within the cell,cause the cell lipid peroxidation,which damaged cells.

Key words: avian influenza virus subtype H9; MDCK cells; antioxidant function

CLC Number:

S855.3

ZHENG Liang-yan, CHEN Long, XU Jia-hua, TANG Zhao-xin. Effect of Avian Influenza Virus Subtype H9 on Antioxidant Function in MDCK Cells[J]. , 2013, 40(11): 175-179.

References

1 王秋林,王浩毅,王树人.氧化应激状态的评价[J]. 中国病理生理杂志,2005(10):2069~2074.
2 赵宝洪,高洪,屈长林,等.自由基检测方法[J]. 动物医学进展,2007(7):107~110.
3 景小冬,章振华,李林,等.H9亚型禽流感病毒流行毒株交叉免疫攻毒保护试验[J]. 中国畜牧兽医,2010,37(6):134~137.
4 Babizhayev M A,Deyev A I. Management of the virulent influenza virus infection by oral formulation of nonhydrolizedcarnosine and isopeptide of carnosine attenuating proinflammatory cytokine-induced nitric oxide production[J]. Am J Ther,2012,19(1):e25~e47.
5 Fang X L,Shu G,Yu J J,et al. The anorexigenic effect of serotonin is mediated by the generation of NADPH oxidase-dependent ROS[J]. PLoS One,2013,8(1):e53142.
6 Grossman C J,Wiseman J,Lucas F S,et al. Inhibition of constitutive and inducible cyclooxygenase activity in human platelets and mononuclear cells by NSAIDs and Cox 2 inhibitors[J]. Inflamm Res,1995,44(6):253~257.
7 Hosakote Y M,Liu T,Castro S M,et al. Respiratory syncytial virus induces oxidative stress by modulating antioxidant enzymes[J]. Am J Respir Cell Mol Biol,2009,41(3):348~357.
8 Huet O,Dupic L,Batteux F,et al. Postresuscitation syndrome: Potential role of hydroxyl radical-induced endothelial cell damage[J]. Crit Care Med,2011,39(7):1712~1720.
9 Oda T,Akaike T,Hamamoto T,et al. Oxygen radicals in influenza-induced pathogenesis and treatment with pyran polymer-conjugated SOD[J]. Science,1989,244(4907):974~976.
10 Shi X,Shi Z,Huang H,et al. Therapeutic effect of recombinant human catalase on H1N1 influenza-induced pneumonia in mice[J]. Inflammation,2010,33(3):166~172.
11 Vlahos R,Stambas J,Selemidis S. Suppressing production of reactive oxygen species (ROS) for influenza A virus therapy[J]. Trends Pharmacol Sci,2012,33(1):3~8.
12 Yang C F,Shen H M,Ong C N. Ebselen induces apoptosis in HepG₂ cells through rapid depletion of intracellular thiols[J]. Arch Biochem Biophys,2000,374(2):142~152.

[1]	WANG Shuaijie, TAN Zihan, LI Hanbo, SUN Xiangxue, AI Sihan, WANG Lei, ZHAO Di, HOU Yongqing. Effects of Quercetin on Intestinal Morphology, Antioxidant Function and Jejunal Lipid Metabolism Gene Expression in Piglets with Porcine Epidemic Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3391-3399.
[2]	ZHANG Yuan, YU Mengmeng, SUN Liuke, GUO Xing, NA Lei, LIU Diqiu, JIANG Qian, ZHANG Haili, WANG Xiaojun. Analysis of Canine ANP32 Protein Polymorphism and Effect on RNA Polymerase Activity of Influenza A Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2462-2473.
[3]	WANG Chaopu, MA Xianyong, TIAN Zhimei, YU Miao, LIU Zhichang, DENG Dun, RONG Ting, TI Junling, SONG Min, ZHANG Huihua. Effects of Compound Chinese Medicine Fermentation Powder on Growth Performance,Nutrient Apparent Digestibility,Antioxidant Function and Immune Function of Weaned Piglets [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1322-1331.
[4]	CHENG Dengfang, MEI Chen, LIU Juan, WANG Hongjun, XIAN Hong. Effect of Chinese Herbal Prescription on the Proliferation of H9N2 in MDCK Cells [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 650-657.
[5]	LIU Jiao, CHANG Wenhuan, CHEN Zhimin, ZHENG Aijuan, LIU Guohua, CAI Huiyi, ZHENG Yi. Effect of Moringa oleifera Lam. Extract on Production Performance,Slaughter Performance,Meat Quality and Serum Biochemical Indexes in AA Broilers [J]. , 2019, 46(2): 414-423.
[6]	MAO Junzhou, DONG Li, WANG Shunan, PENG Zhong, ZHONG Zhaoxin, YU Lihuai. Effects of Enterococcus faecium on Antioxidant Function in Serum and Liver of Arbor Acres Broilers [J]. , 2018, 45(8): 2182-2189.
[7]	QIAO Hongxing, SHI Hongtao, SONG Yuzhen, ZHAO Shengzhen, BIAN Chuanzhou. Effects of Fermented Astragalus on Growth Performance and Antioxidant Function of Broilers [J]. , 2018, 45(3): 705-711.
[8]	CHEN Ning-bo, ZHANG Chong-yu, JIANG Shu-zhen, Huang Li-bo, CHEN Xiang-xing, SUN Jian-li, YANG Wei-ren. Effects of Fusarium Toxins on Serum Enzymes Activity,Liver Antioxidant Function and Histopathology of Weaned Gilts [J]. , 2015, 42(9): 2384-2390.
[9]	LIU Xiao-jun, WANG Yong-sheng, LIU Yan-fen. Effects of Leaves and Seeds of S.album on Growth Performance and Antioxidant Function of Wenchang Chickens [J]. , 2013, 40(8): 54-56.
[10]	REN Chang-bao;HU Kai;YAN Cheng;SU Rong-sheng;GUO Jian-ying;PAN Jia-qiang;TANG Zhao-xin;. Effect of High-copper Diets on Liver Antioxidant Function and Liver Cell Apoptosis of Broilers [J]. , 2011, 38(11): 18-22.
[11]	GAO Yan-ni;YUAN Ying;WANG Ji-lei;ZHAI Feng. Effect of Concentration Echinacea on Antioxidant Function of Broilers [J]. , 2008, 35(6): 18-20.
[12]	CAO Guodi;ZHAO Hengshou. Recent Advances in the Effect of Vitamin E on Immune and Antioxident Function in Poultry [J]. , 2007, 34(1): 34-36.

Effect of Avian Influenza Virus Subtype H9 on Antioxidant Function in MDCK Cells

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 12

Recommended Articles

Metrics

Comments