抗三肽囊素单克隆抗体的制备

Abstract

Abstract: In order to produce monoclonal antibodies (McAbs) against bursin, the artificial antigens of bursin (BSA-KHG-NH₂and OVA-KHG-NH₂) were prepared by EDC method, in which the amino group on -Lys of bursin molecules was conjugated to bovine serum albumin (BSA) or chicken ovalbumin (OVA), respectively. BALB/c mice were immunized four times by BSA-KHG-NH₂, and eighteen hybridoms producing anti-bursin monoclonal antibodies were obtained by limited dilution. The binding of artificial antigen OVA-KHG-NH₂ to the McAbs were competitively inhibited by free bursin(KHG-NH₂) and GKHG-NH₂peptide,respectively, but couldn't be inhibited by free KHGK peptide. These results indicated that the 18 McAbs specifically bonded to bursin molecules. An IC-ELISA method to detect bursin was developed using McAb 2D2, in which the detection limits was about 4 ng/mL and the linear range of the inhibition curve was about 4 to 500 ng/mL, the recovery rate of bursin in the samples ranged from 85.0% to 92.4%.

Key words: bursin; artificial antigen; monoclonal antibodies; IC-ELISA

CLC Number:

S852.4⁺3

DENG Shun-zhou, ZHENG Zhi-gang, ZHANG Wen-bo, XIE Xiao-fang, ZOU Liu, WU Xiang-dong, CHEN Nan-hui, HE Hou-jun, ZHU Zhi-xiu. Preparation of Monoclonal Antibodies against Bursin[J]. , 2013, 40(10): 76-80.

References

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Preparation of Monoclonal Antibodies against Bursin

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