16S rRNA甲基化酶在鸡肠道大肠杆菌中传播方式的分析

Abstract

Abstract: To study the transfer mode of rmtB and armA in chicken intestinal E.coli,plasmid conjugation experiment was carried out to study the horizontal transfer mode of rmtB and armA genes, and conjugative transfer frequencies were calculated. Broth microdilution method was applied to test susceptibility of 16S rRNA emthylase-positive isolates and their transconjugants to antimicrobial agents. As a result, all the rmtB genes located in five rmtB-positive isolates were easily transferred to recipients of E.coli C600. Conjugative transfer frequencies varied from 4.30×10^-10 to 5.5×10^-6 per recipient cell, and the armA gene located in one armA-positive isolate was not easily transferred to the recipient of E.coli C600. The results suggested that horizontal transfer was responsible for the dissemination of rmtB gene in chicken intestinal E.coli, but not for armA.

Key words: 16S rRNA methylase; plasmid; horizontal transfer; resistance

CLC Number:

S859.7

DING Xiao-li, CHEN Lin, HUANG Dong-zhang, LIU Jun-dong, WEI Dong-xia, GUAN Yuan-hong, LI Ju-yin, ZHOU Si-si, CHEN Xin-xiu. Analysis of Transfer Modes of 16S rRNA Methylase in Chicken Intestinal E.coli [J]. , 2013, 40(10): 216-220.

References

1 刘志远,许淑珍,马纪平. 高耐庆大霉素粪肠球菌质粒接合转移试验的方法探讨[J].中华医院感染学杂志,2005,5(6): 608~610.
2 陈琳,刘健华,张俊丰,等.16S rRNA甲基化酶RmtB在猪肠道菌中的传播方式分析[J].中国畜牧兽医,2009,36(12):31~35.
3 林宁,孙海平,糜祖煌.多重耐药大肠埃希菌16S rRNA甲基化酶、氨基糖苷类修饰酶基因研究[J].中国抗生素杂志,2008,33(9):536~539.
4 Bogaerts P,Galimand M,Bauraing C,et al. Emergence of ArmA and RmtB aminoglycoside resistance 16S rRNA methylases in Belgium[J].J Antimicrob Chemother,2007,59(3):459~464.
5 Chen L,Chen Z L,Liu J H,et al. Emergence of RmtB methylase-producing Escherichia coli and Enterobacter cloacae isolates from pigs in China[J].J Antimicrob Chemother,2007,59(5):880~885.
6 Davis M A,Baker K N K,Orfe L H,et al. Discovery of a gene conferring multiple-aminoglycoside resistance in Escherichia coli[J].Antimicrobial Agents and Chemotherapy,2010,54(6):2666~2669.
7 Doi Y,Yokoyama K,Yamane K,et al.Plasmid-mediated 16S rRNA methylase in Serratia marcescens conferring high-level resistance to aminoglycosides[J].Antimicrob Agents Chemother,2004,48(2):491~496.
8 Doi Y,de Oliveira Garcia D,Adams J,et al. Coproduction of novel 16S rRNA methylase RmtD and metallo-beta-lactamase SPM-1 in a panresistant Pseudomonas aeruginosa isolate from Brazil[J].Antimicrob Agents Chemother,2007,51(3):852~856.
9 Doi Y,Adams-Haduch J M,Paterson D L. Genetic environment of 16S rRNA methylase gene rmtD[J]. Antimicrobial Agents and Chemotherapy,2008,52(6):2270~2272.
10 Galimand M,Courvalin P,Lambert T. Plasmid-mediated high-level resistance to aminoglycosides in Enterobacteriaceae due to 16S rRNA methylation[J].Antimicrob Agents Chemother,2003,47(8):2565~2571.
11 Galimand M,Abtcheva S,Courvalin P,et al. Worldwide disseminated armA aminoglycoside resistance methylase gene is borne by composite transposon Tn1548[J].Antimicrob Agents Chemother,2005,49(7):2949~2953.
12 Galimand M, Courvalin P, Lambertb T. RmtF, a new member of the aminoglycoside resistance 16S rRNA N7 G1405 methyltransferase family[J], Antimicrob Agents Chemother,2012,56(7):3960~3962.
13 Gonzalez-Zorn B,Catalan A,Escudero J A,et al. Genetic basis for dissemination of armA[J].J Antimicrob Chemother,2005,56(3):583~585.
14 Lee H,Yong D,Yum J H,et al. Dissemination of 16S rRNA methylase-mediated highly amikacin-resistant isolates of Klebsiella pneumoniae and Acinetobacter baumannii in Korea[J].Diagn Microbiol Infect Dis,2006,56(3):305~312.
15 Leung K S. Antibiotics:Action and resisitance in gram-negative bacteria[J].The Journal of Microbiology, Immunology and Infection,2002,35:1~11.
16 Schwarz S, Kehrenberg C, Walsh T R. Use of antimicrobial agents in veterinary medicine and food animal production[J]. International Journal of Antimicrobial Agents,2001,17:431~437.
17 Sheng J F,Li J J,Tu S,et al.Bla_KPC and rmtB on a singleplasmid in Enterobacter amniges and Klebsiella pneumoniae isolates from the same patient[J]. Eur J Clin Microbiol InfectDis,2012,31(7):1585~1591.
18 Tijet N,Andres P,Chung C,et al. RmtD2, a new allele of a 16S rRNA methylase gene, has been present in Enterobacteriaceae isolates from argentina for more than a decade[J]. Antimicrobial Agents and Chemotherapy,2011,55(2):904~909.
19 Wachino J,Yamane K,Shibayamak K,et al. Novel plasmid-mediated 16S rRNA methylase,RmtC,found in a Proteus mirabilis isolate demonstrating extraordinary high-level resistance against various aminoglycosides[J].Antimicrobial Agents and Chemotherapy,2006,50(1):178~184.
20 Wachino J,Shibayama K,Kurokawa H,et al. Novel plasmid-mediated 16S rRNA m1A1408 methyltransferase, NpmA, found in a clinically isolated Escherichia coli strain resistant to structurally diverse aminoglycosides[J].Antimicrobial Agents and Chemotherapy,2007,51(12):4401~4409.
21 Yokoyama K,Doi Y,Yamane K,et al. Acquisition of 16S rRNA methylase gene in Pseudomonas aeruginosa[J].Lancet,2003, 362(9399):1888~1893.

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Analysis of Transfer Modes of 16S rRNA Methylase in Chicken Intestinal E.coli

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