Abstract: In order to obtain the monoclonal antibody (McAb) against CA protein of JSRV-NM strain, BALB/c mice were immunized with purified CA fusion protein expressed in E.coli. Myeloma cells SP2/0 were fused with the splenocytes of the fore times immunized mice by hybridomas technique, and six specific antibody-producing hybridomas were screened by indirect ELISA with CA fusion protein. Five hybridomas cells of them could product McAb steadily after 3 cycles of cloning, and three McAbs of them showed positive reaction to the CA protein expressed in eukaryotic cells by indirect immunefluorescence tests and western blotting analysis.The results indicated that we had obtained three hybridomas cells which could product specific McAb against CA protein of JSRV-NM strain steadily. The McAb against CA protein of JSRV-NM strain developed would be useful as a basis of diagnosis and epitope identification.

Key words: jaagsiekte sheep retrovirus(JSRV); capsid protein; hybridomas technique; ELISA; indirect immunefluorescence