东方田鼠组织与血清中抗日本血吸虫相关蛋白的分离与筛选

Abstract

Abstract: In order to comprehensive compare and screen potential proteins resistance to Schistosoma japonicum infection from tissues and serum of Microtus fortis, the heart, liver, spleen, lung, kidney, muscle, skin, bone marrow, brain tissue and serum protein of Microtus fortis were collected respectively. The same proteins obtained from rabbits, cattle and mice infected with Schistosoma japonicum as control. The individual soluble proteins were separated by SDS-PAGE and stained with kaomasi blue R-250. The band sizes and their differences were analyzed. Results showed that the 85 ku proteins fractions in serum was more obviously and highly expressed after 12, 45 days post-infection in mice than in Microtus fortis; the 135 ku proteins fractions in liver and serum of Microtus fortis was characterized and more highly expressed than other animals. It maybe an important effective molecule against Schistosoma japonicum infection in Microtus fortis.

Key words: Microtus fortis; Schistosoma japonicum; separation and screening

CLC Number:

CHENG Gang, XU Chun-juan, WANG Jing-ren, LI Shu-hong, ZENG Wen-hu. Separation and Screening Proteins Associated with Resistant to Schistosoma japonicum Infection from Tissues and Serum of Microtus fortis[J]. , 2013, 40(1): 33-35.

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References

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Separation and Screening Proteins Associated with Resistant to Schistosoma japonicum Infection from Tissues and Serum of Microtus fortis

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[1]	WANG Saiqiao, ZHAO Lu, ZHAI Zhenhan, ZHANG Binglei, JIA Wanhang, WANG Yuqin. Tissue Expression and Bioinformatics Analysis of miR-449a/b Precursor Sequences in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2175-2184.
[2]	WEI Mingbang, BIANBA Qiongda, XIAO Qingqing, DUAN Mengqi, CHAMBA Yangzom, SHANG Peng. Cloning,Bioinformatics and Expression Analysis of CDKN1B Gene in Tibetan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2196-2206.
[3]	LI Jie, CHEN Chuwen, ZHAO Ruipeng, LIU Yuan, LI Zhixiong. Research Progress on Long Non-coding RNA of Muscle Development in Livestock and Poultry [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2427-2438.
[4]	ZHANG Dianqi, MA Xinhao, YANG Zhimei, MEI Chugang, ZAN Linsen. Bioinformatics Analysis of PAI1 Gene and Its Effect on the Proliferation and Differentiation of Intramuscular Adipocytes in Qinchuan Beef Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1729-1741.
[5]	WANG Haorui, YU Baojun, YANG Jiayi, WEI Rong, FU Xi, WANG Chuanchuan, ZHANG Di, LI Desheng, CAI Zhengyun, GU Yaling, ZHANG Juan. Bioinformatics Analysis and Tissue Expression of DERA Gene in Jingyuan Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1764-1773.
[6]	WU Zhijuan, CHAI Zhixin, WANG Jikun, WANG Jiabo, ZHONG Jincheng, XIN Jinwei. Expression Analysis of Specific Matrix Metalloproteinases and Functionally Related Genes of Skeletal Muscles in Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1774-1784.
[7]	PAN Pengcheng, LEI Zongquan, LI Xian, HU Xiangyun, QIN Qiantao, QIN Zhaoxian, GUAN Zhihui, CHEN Baojian, XIE Bingkun. Bioinformatics Analysis,Eukaryotic Expression Vector Construction and Tissue Expression of MYL2 Gene in Luchuan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1796-1806.
[8]	LIU Jialong, ZHANG Yiwen, ZHANG Yueran, SUN Qijuan, CHEN Jian, HE Lei, JIA Yanyan, DING Ke, YU Zuhua. Cloning,Prokaryotic Expression and Bioinformatics Analysis of ELAVL1 Gene in Chicken [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1807-1817.
[9]	ZHUO Na, ZHU Zhongwu, LI Jing, HU Shixiang, WANG Jianchang, LIN Hua, CHEN Chaolin, HAN Diangang, AI Jun, CHEN Peifu. Bioinformatics Analysis and Eukaryotic Expression of ORF2 Protein of Swine Hepatitis E Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1959-1970.
[10]	LI Dan, ZHANG Haisen, WANG Yiqun, GAO Dengke, ZHAO Hongcong, LI Chao, JIN Yaping, CHEN Huatao. Eukaryotic Expression Vector Construction,Bioinformatics and Tissue Expression Profiles Analysis of CRY1 Gene in Dairy Cows [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 870-881.
[11]	CHANG Yitong, ZHANG Wei, PENG Yinglin, CHEN Chen. Evolutionary Analysis,Target Gene Prediction and Tissue Expression Analysis of miR-192 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 882-892.
[12]	ZHAO Yu, ZHAO Ruli, GAO Yuan. Analysis of Codon Preference in Camelus ferus Genome [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 429-439.
[13]	WEI Yirong, ZHENG Zihua, ZHANG Sanbao, SONG Ying, JIANG Haiyu, SUN Wenyue, CHENG Pengfei, LIU Yufan, ZOU Jianwei, HUANG Yanna, PAN Yan, JIANG Qinyang. Cloning,Bioinformatics Analysis and Tissue Expression of UCP1 Gene in Nubian Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 440-450.
[14]	CHUI Linya, XIA Boce, HUANG Jiaojiao. Cloning, Bioinformatics and Expression Analysis of DJ-1 Gene in Large White×Landrace Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4513-4523.
[15]	LI Xiaojiao, HE Yanhua, ZHU Xinyu, ZOU Xian, LUO Chenglong. Research Progress on Application of CRISPR/Cas9 Technology in Pigs and Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4665-4673.