东方田鼠组织与血清中抗日本血吸虫相关蛋白的分离与筛选

Abstract

Abstract: In order to comprehensive compare and screen potential proteins resistance to Schistosoma japonicum infection from tissues and serum of Microtus fortis, the heart, liver, spleen, lung, kidney, muscle, skin, bone marrow, brain tissue and serum protein of Microtus fortis were collected respectively. The same proteins obtained from rabbits, cattle and mice infected with Schistosoma japonicum as control. The individual soluble proteins were separated by SDS-PAGE and stained with kaomasi blue R-250. The band sizes and their differences were analyzed. Results showed that the 85 ku proteins fractions in serum was more obviously and highly expressed after 12, 45 days post-infection in mice than in Microtus fortis; the 135 ku proteins fractions in liver and serum of Microtus fortis was characterized and more highly expressed than other animals. It maybe an important effective molecule against Schistosoma japonicum infection in Microtus fortis.

Key words: Microtus fortis; Schistosoma japonicum; separation and screening

CLC Number:

CHENG Gang, XU Chun-juan, WANG Jing-ren, LI Shu-hong, ZENG Wen-hu. Separation and Screening Proteins Associated with Resistant to Schistosoma japonicum Infection from Tissues and Serum of Microtus fortis[J]. China Animal Husbandry & Veterinary Medicine, 2013, 40(1): 33-35.

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References

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Separation and Screening Proteins Associated with Resistant to Schistosoma japonicum Infection from Tissues and Serum of Microtus fortis

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[1]	AI Xiaonan, CHENG Lifen, BAI Pu, CHEN Zhenghao, XUE Lina, ZHOU Shenghua. Cloning and Bioinformatics Analysis of ACSL1 Gene and Its Expression Pattern During Adipocyte Differentiation in Lingqiu Qingbei Goats [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(2): 499-511.
[2]	LEI Zhigang, PAN Hong, ZHANG Dandan, LIU Quanhui, SUN Zhe, DENG Shan, HUANG Ben. Cloning, Bioinformatics Analysis and Eukaryotic Expression Vector Construction of MAPK8 Gene in Mashan Black Goats [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(2): 534-544.
[3]	FENG Zhiya, PENG Wanwan, ZHANG Jianping, WU Zhenhui, ZHANG Nan, LI Shuwei, SHI Ruijun. Cloning and Bioinformatics Analysis of KRT79 Gene,and the Effects of Androgens on Its Expression and Distribution of Skin in Hetian Sheep [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(2): 562-573.
[4]	XIE Beiyiting, WANG Yue, MENG Chunhua, QIAN Yong, ZHANG Jun, ZHANG Jianli, WANG Huili, CAO Shaoxian, LI Yinxia. Cloning and Sequence Analysis of PSMB9 Gene in Hu Sheep and Its Effect on Myoblast Proliferation [J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(1): 1-12.
[5]	CHEN Yang, XIANG Yanhua, LI Yihai, HE Xiumiao. Cloning，Expression，Enzyme Activity and Bioinformatics Analysis of Caspase 3 Gene in Chicken [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(10): 4200-4210.
[6]	MAJianqing, SONG Pengyan, YANG Qingfang, KONGJianjun, SONG Zhanfeng, ZHANG Yali, WU Dongwei, XU Zengnian, ZHAO Ning, ZHOU Rongyan, WU Zhanyong. Target Gene Prediction and Bioinformatics Analysis of miR-141 in Capra hircus [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(10): 4211-4221.
[7]	ZHOU Fangting. Cloning,Molecular Characteristics and Tissue Differential Expression Analysis of KLF15 Gene in Buffalo [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(9): 3715-3725.
[8]	LI Jinnan, WANG Yahui, DENG Tianyu, LIANG Mang, DU Lili, LI Keanning, XUE Qingqing, QIAN Li, GAO Xue, ZHANG Lupei, ZHU Bo, CHEN Yan, WANG Zezhao, LI Junya, GAO Huijiang. Cloning,Bioinformatics Analysis and Tissue Expression Profile of Coding Region of Bovine MED28 Gene [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(8): 3225-3236.
[9]	ZHAO Yutian, CHEN Fuju, YI Pingchang, MA Min, WANG Shouning, QUAN Qishiliu. Exploring Candidate Genes Related to Kidney-adjacent Lipid Deposition in Yak Calves Based on Transcriptome Sequencing [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(7): 2727-2738.
[10]	JIANG Lei, WANG Yuxin, WAN Yuan, ZAN Linsen, WANG Hongbao. Bioinformatics and Expression Characteristics Analysis of MGP Gene in Qinchuan Cattle [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(6): 2261-2272.
[11]	WEI Wei, GAO Zihan, YUE Miao, ZUO Yang, WANG Tonggang, WEI Fulei, YU Luxian, LIANG Jian. Cloning of the SMIT1 and SMIT2 Genes in Gymnocypris przewalskii and Its Response to Alkaline Environment [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(6): 2285-2299.
[12]	LU Qingwei, TANG Sen, LI Bin, QIN Chongkai, LIU Wenna, PU Xue, WANG Yaqian, WU Cuiling, FU Xuefeng. Screening of circRNA Related to Secondary Hair Follicle Cycle Development in Jiangnan Cashmere Goats Based on Transcriptome Sequencing [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(5): 1793-1806.
[13]	HUANG Qiaoyan, LI Wei, WANG Xinkun, XING Feng. Cloning,Bioinformatics and Tissue Differential Expression Analysis of PROKR2 Gene in Dolang Sheep [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(4): 1339-1348.
[14]	WANG Yan, ZHANG Ying, ZHAO Yani, YI Lin, SHI Zhen, ZHOU Changming, ZHOU Wanrong, JIANG Lili, FAN Zhaobin. Cloning,Bioinformatics and Tissue Expression Characteristics of STING Gene in White Leghorn Chickens [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(4): 1372-1381.
[15]	YANG Yaqiong, CHENG Hongxing, LIANG Mingxia, LIU Yulan, FU Shulin, ZHANG Jing, CHEN Hongbo, REN Hongyan, GUO Ling, CHAO Zhe. Integrated Validation for DNA Methylation and mRNA Expression of 4 Genes in Brain of Glaessrerlla parasuis-infected Piglets [J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(4): 1651-1659.