快速鉴定猪链球菌和马链球菌兽疫亚种双重PCR方法的建立

Abstract

Abstract: To establish a rapid, specific and sensitive double PCR identification method of Streptococcus suis and Streptococcus equi subspecies pathogen,primers were designed based on gene conserved region of the M-like protein of Streptococcus equi subspecies and GDH protein of Streptococcus suis. The amount of M-like and GDH primers were both 1 μL (20 pmol/L). The optimal annealing temperature was 52.3℃. The sensitivity of double PCR were 10 CFU for Streptococcus suis and 100 CFU for Streptococcus equi subspecies. The specific trials had shown that five kinds of common pathogens had non-specific bands appeared in the double PCR system. 1 Streptococcus suis and 2 Streptococcus equi subspecies were detected by this method.

Key words: Streptococcus suis; Streptococcus equi subspecies; double PCR

CLC Number:

LI Shu-guang, LI Tian-zhi, LI Feng, HU Lin-lin, SHEN Zhi-qiang. Establishment of Double PCR for Rapid Identification of Streptococcus suis and Streptococcus equi Subspecies[J]. China Animal Husbandry & Veterinary Medicine, 2013, (7): 43-46.

References

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Establishment of Double PCR for Rapid Identification of Streptococcus suis and Streptococcus equi Subspecies

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