小鼠髓源未成熟树突状细胞的分离与培养

Abstract

Abstract: To establish a method of isolation, cultivation and purification of dendritic cells from mouse bone marrow in vitro. Mononuclear cells isolated form mouse bone marrow were induced into immature dendritic cells by being cultured with rGM-CSF and then examined from aspects of morphology, phenotype and function of T lymphocyte proliferation. The result showed that a large number of typical dendritic cells were observed after culturing for 9 days, and high expressed CD11c 81.09%, middle exproessed MHCⅡ, low expressed CD40, CD80, CD86. A method to generate large number of DCs from mouse bone marrow in vitro had been established.

Key words: mouse; dendritic cells; bone marrow

CLC Number:

Q813.1

LIAO Juan-hong, LIN Xiang-mei, JIA Guang-le. Isolation and Culture of Mouse Bone Marrow-derived Dendritic Cells[J]. , 2013, (7): 107-111.

References

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Isolation and Culture of Mouse Bone Marrow-derived Dendritic Cells

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