马绒毛膜促性腺激素单克隆抗体的制备及鉴定

Abstract

Abstract: This study fusing the spleen cells of BALB/c mice which was immunized with high purified equine chorionic gonadotropin and myeloma cells SP2/0 at the rate of 1∶10, and screening the positive cloning by the means of indirect enzyme linked immunosorbent assay. Positive cells were clone through limited dilution method, injecting the mixture into the abdominal cavity of mice after intermediate cultured. Antibody titer was assayed by ways of indirect ELISA. Eight hybridoma cell lines were obtained which could secrete eCG stably, titer reached above 10^-5. The obtained eCG hybridoma cell lines, which laid a foundation to assay the level of eCG.

Key words: equine chorionic gonadotropin; hybridoma cell line; monoclonal antibody; enzyme linked immunesorbent assay

CLC Number:

R392.11

SU Xiao-qian, DENG Liang, JIA Yu, HAN Guo-cai. Preparation and Identification of Monoclonal Antibody Against Equine Chorionic Gonadotropin[J]. .

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Preparation and Identification of Monoclonal Antibody Against Equine Chorionic Gonadotropin

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