猪β-防御素-2转化酿酒酵母方法的比较分析

Abstract

Abstract: In order to develop a high efficiency genetic transformation system for Saccharomyces cerevisiae, two different transformation methods were compared in this study, which were electroporation transformation and lithium acetate transformation. The results showed that two methods all had strict request to cell growth state. For lithium acetate transformation, under the conditions of D_{600 nm} was 1.051, were treated for 60 min by LiAc, transformation efficiency was 136/μg DNA. While for electroporation transformation, 154/μg DNA were detected under the conditions of D_{600 nm} was 1.359, voltage of 2.5 kV. All results demonstrated that LiAc transformation method would be beneficial to develop the genetic transformation system for Saccharomyces cerevisiae.

Key words: β-defensin-2; Saccharomyces cerevisiae; transformation

CLC Number:

S859.79⁺7

WU Ya-kun, PENG Zi-xin, FENG Qiu-yue, XIE Fei, LIU Peng, WANG An-ru. Comparison Analysis of Two Methods of Porcine-β-defensin-2 Gene Transforming into Saccharomyces cerevisiae[J]. , 2012, 39(7): 138-141.

References

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Comparison Analysis of Two Methods of Porcine-β-defensin-2 Gene Transforming into Saccharomyces cerevisiae

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