伊维菌素小单室脂质体工艺配方优化及质量分析

Abstract

Abstract: To obtain high entrapment rate of ivermectin single unilamellar vesicles. The study was aim to optimizing preparation process of ivermectin single unilamellar vesicles; we had selected some factors such as ultrasonic frequency, ultrasonic time, mass ratio of lecithin to cholesterol and mass ratio of ivermectin to lecithin to design orthogonal test L₈(2⁷), and we used RP-HPLC to determine the entrapment rate of ivermectin single unilamellar vesicles under every combination of process and formula. The results showed the optimal process and formula combination of ivermectin single unilamellar vesicles were as follows: the ultrasonic frequency was 200 kHz, the ultrasonic time was 6 minutes and the mass ratio of ivermectin to lecithin was 1:10. We have obtained high qualitative ivermectin single unilamellar vesicles by optimization,the loading rate reach (92.35?0.61)%,the quality of drug is fine, the preparation process are simple and feasible.

Key words: ivermectin; single unilamellar vesicles; prescription and preparation technology; quality analyzing; orthogonal test

CLC Number:

S859.79⁺5

HE Shu-hai, BASANG Wang-dui, SHE Yong-xin. Optimizing Process and Formula and Analyzing Quality for Ivermectin Single Unilamellar Vesicles[J]. , 2012, 39(7): 108-112.

References

1 刘晓谦,王锦玉,仝燕,等. 脂质体制备技术及其研究进展[J].中国药学杂志,2011,46(14):1084~1088.
2 刘根新,张继瑜,刘英,等.HPLC法测定微乳中伊维菌素的含量[J].中国兽药杂志,2008,42(11):34~36.
3 朱晓娟,李引乾,侯勃,等.伊维菌素脂质体的制备及其质量评价[J].西北农林科技大学学报(自然科学版),2010,38(4):24~30.
4 许忠华,张洪波. 超声清洗的空化作用机理[J].哈尔滨铁道科技,2009,21(4):110~112.
5 邬瑞光,李维峰,侯俊玲,等.植物固醇在脂质体中的应用研究进展[J].中国新药杂志,2011,20(12):1092~1094.
6 佘永新,巴桑旺堆,何书海,等. 高效液相色谱法测定伊维菌素脂质体药物的含量及包封率[J].中国畜牧兽医,2010,37(4):80~84.
7 张艳晶,杨强,马艳铃,等. 苹果酸舒尼替尼脂质体药物含量及包封率的测定[J].药物分析杂志, 2011,31(7):1209~1212.
8 张婵,郑爽英. 超声空化作用及应用[J].水力资源与工程学报,2009,20(1):136~138.
9 李发新,李春秀,常华山,等. 不同剂量的伊维菌素注射剂和浇泼剂对天祝牦牛牛皮蝇防制效果观察[J].畜牧兽医杂志,2011,30(3):20~21.
10 陈谦,邹欣晔,程建春. 超声波声孔效应中气泡动力学的研究[J].物理学报,2006,55(12): 6476~6479.
11 林俏慧,缪珑,潘力,等. 抗体交联体的制备[J].华南理工大学学报(自然科学版),2003,31(9):76~80.
12 徐霞,刘增再,刘毅,等. 伊维菌素脂质体的制备工艺[J].湖南农业大学学报(自然科学版),2005,31(3):300~303.
13 蒋晨阳,石玉生,蔡进忠. 液相色谱法测定伊维菌素泼浇剂中伊维菌素和氮酮的含量[J].黑龙江畜牧兽医,2005(2):52~437.
14 蔡泽川,张浩,徐发荣,等. 长效伊维菌素注射剂对北京房山区绒山羊寄生虫病的疗效观察[J]. 黑龙江畜牧兽医,2011, 26 (6): 140~142.
15 穆筱梅,钟振声. 维生素E、维生素C 和BHT对大豆磷脂脂质体的抗氧化作用[J]. 大豆科学,2006 (4):434~437.
16 Ardnzazu G C,Ana M S P,M Jose D L,et al.The pharmacoki-netics and metabolism of ivermectin in domestic animal species[J].The Veterinary Journal,2009,179(1):25~37.
17 Hans S,et a1.Assessm ent of the environmental fate and effects of ivermectin in aquatic mesocosms[J].Aquatic Toxicology,2007,85:229~240.
18 Õmura S.Ivermectin:25 years and still going strong[J].Inter-national Journal of Antimicrobial Agents,2008,31(2):91~98.

[1]	CHEN Yuxiao, LI Huanjuan, XI Xiaohua, HAO Jie, ZHOU Degang, SI Hongbin. Study on Extraction Process of Jiawei Pingwei Oral Liquid [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2414-2422.
[2]	ZHU Yixuan, LIU Shipeng, ZHANG Wei, LI Jinrong, PENG Fei, LIN Na, WANG Yuyan, LIU Dandan. Study on the Optimization of Modified Formula for Inhibiting Streptococcus equi subspecies equi and Its Bacteriostatic Effect in Vivo [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 4085-4096.
[3]	YANG Shuai, ZHOU Di, ZHANG Xinxin, HU Xiangwei, YANG Liguo. Optimization of Cryoprotectant for Attenuated Salmonella Cholerae C500 [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3696-3704.
[4]	LIU Yingying, YUE Zhiguang, WU Ningpeng, LIU Zhenzhen, YAN Yaqun, ZHANG Sumei, JIAN Fuchun, ZHANG Longxian, WANG Rongjun, NING Changshen. Comparative Study on Pharmacokinetics of Long-acting Transdermal Agents of Ivermectin and Common Injection in Rabbits [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 267-274.
[5]	SUN Jing, YE Dongyang, WANG Xurong, HOU Yanhua, WANG Lei, ZHANG Jingyan, ZHANG Kai, ZHANG Kang, LI Jianxi, YANG Zhiqiang. Study on Screening of Traditional Chinese Medicine Formula and Its Anti-bacterial Effects Preventing Bovine Mastitis [J]. , 2018, 45(7): 1990-2000.
[6]	WANG Si-han, ZHANG Ji-yu, XING Shou-ye, LI Bing, ZHOU Xu-zheng. Content Determination of Ivermectin in the Ivermectin Microemulsion Injection [J]. , 2017, 44(6): 1861-1868.
[7]	TIAN Qi-zhen, CAI Bing-yan, YUAN Wei-feng. Efficacy of Chinese Herbal Medicine Canine Acariasis Spirit on Demodex canis [J]. , 2017, 44(11): 3358-3364.
[8]	LU Guang-fu, CHEN Xiao-lan, HUANG Ya-qi, JIANG Chun-mao, YU Jie, QU Jing-wen, GENG Qian-li. Study on the Optimum Extraction Process of Mulberry Leaf Polysaccharide [J]. , 2016, 43(7): 1922-1927.
[9]	XING Shou-ye, ZHOU Xu-zheng, LI Bing, NIU Jian-rong, WEI Xiao-juan, ZHANG Ji-yu. Study on Sterility Test Method of Ivermectin Microemulsion [J]. , 2016, 43(1): 256-260.
[10]	DING Dan, SHENG Xiao-li, LIANG Kong-xian, XU Qi, LIU Wei. Study on Ivermectin Nanoemulsion for Transdermal Drug Delivery [J]. , 2015, 42(2): 401-407.
[11]	XING Shou-ye, ZHOU Xu-zheng, LI Bing, NIU Jian-rong, WEI Xiao-juan, ZHANG Ji-yu. Allergic Study of Ivermectin Microemulsion [J]. , 2014, 41(11): 275-278.
[12]	NING Yu-chang;YANG Xiang-ke. Preparation of Microcapsules and Characteristic Research of Lactobacillus from Chicken [J]. , 2011, 38(2): 220-223.
[13]	ZHANG Rui-li;CUI Liang-Liang;HAO Zhi-hui;ZHAO Yong-da. Compare Pharmacokinetics of 5 Different Ivermectin Injection in Rabbit [J]. , 2011, 38(11): 188-190.
[14]	JIANG Xiao-wen;ZHANG Chun-yan;YU Wen-hui;XU Li. Study on Pharmacokinetics of Ivermectin Emulsion Compound in Goats [J]. , 2010, 37(8): 169-171.
[15]	JIANG Xiao-wen;FENG Guo-feng;XU Ming-xing. Effects of Ivermectin on Male Rats Procreation [J]. , 2010, 37(6): 174-175.

Optimizing Process and Formula and Analyzing Quality for Ivermectin Single Unilamellar Vesicles

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments