猪源戊型肝炎病毒ORF2部分基因的原核表达及其鉴定

Abstract

Abstract: The assay was aim to express swine hepatitis E virus (sHEV) ORF2 partial gene fragments, purify the expression product and analyze the antigenicity. ORF2 gene was amplified by PCR from sHEV cDNA as a template,and the recombinant plasmid pET32a-dORF2 was constructed,then transformed into the expression bacteria BL21. After IPTG induction, the protein was analyzed by SDS-PAGE and Western blotting. The results showed that the protein was expressed from ORF2 gene in the form of soluble, the protein was 60 ku. The result of Western blotting analysis indicated that the protein could react with the HEV positive serum.

Key words: hepatitis E virus; ORF2 gene; recombinant plasmid; prokaryotic expression

CLC Number:

OUYANG Yun, ZHANG Liang-quan, LIU Zhi-gang, QI Hai-tao, XU Ting-chuan, ZHANG Min-ze, ZHANG Chao-yi, ZHANG Gui-hong. Swine Hepatitis E Virus ORF2 Partial Gene Expression and Identification of Prokaryotic[J]. , 2012, 39(6): 86-89.

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Swine Hepatitis E Virus ORF2 Partial Gene Expression and Identification of Prokaryotic

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