Abstract: To develop the monoclonal antibody against chronic wasting disease, in this study, the PrP^c-null mice were immunized with elk purified recombinant prion protein(PrP^c). After twice booster immunizations, mouse spleen cells were fused with myelomas SP2/0 by lymphocyte hybridoma technique. Five hybridoma cell strains which could stably excrete specific monoclonal antibodies against elk PrP^c were obtained by ELISA and three times subclone. The five hybridoma cell strains were named 5A5,3B2,6D12,5E3 and 1F5 respectively, whose ELISA titer were all more than 1∶10000. By evaluating, three strains belonged to IgG1 subset and other two were IgG2a subset. These McAbs could identify both elk recombinant PrP^c and PrP^c from elk healthy brain homogenate by Western blotting. This study filled the void of monoclonal antibody against CWD in China. Furthermore, the monoclonal antibodies laid a foundation for the research and detection of CWD.

Key words: elk; recombinant PrP^c; monoclonal antibody; Western blotting