禽流感病毒A/duck/Fujian/31/2007(H5N1)株M1 基质蛋白的原核表达与免疫原性鉴定

Abstract

Abstract: Based on published AIV(H5N1)genome sequence, a fragment of about 750 bp long was amplified by PCR technique with specific primers using biological software DNAStar to analysis. Then the amplified product was directionally cloning into pET30a expression vector. After identifying with enzyme cut and sequencing, the recombinant plasmid was transformed into E. coli BL21(DE3). The recombinant protein M1 was expressed in inclusion body form in E coli after induction with IPTG. After denaturation, purification and renaturation, the concentration of purified protein was 0.656 mg/mL, Western blotting showed the recombinant protein had a good immunogenicity. The purified protein immunized BALB/c mice, indirect ELISA showed that the recombinant protein could produce specific antibodies anti-M1 IgG, which had a good immunogenicity. That is a better basic for diagnostic and genetically engineering vaccine research on H5N1 influenza virus.

Key words: H5N1 influenza virus; matrix protein M1; prokaryotic expression; immunogenicity

CLC Number:

BU Ri-e, WU Jin-hua, SUN Li-jie, LI Ming-gang, LIU Yang, XUE Xiao-yang. Immunogenicity Identification of M1 Matrix Protein of A/duck/Fujian/31/2007 H5N1 Subtype Influenza Virus Expressed in Escherichia coli[J]. , 2012, 39(6): 46-49.

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Immunogenicity Identification of M1 Matrix Protein of A/duck/Fujian/31/2007 H5N1 Subtype Influenza Virus Expressed in Escherichia coli

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