Abstract: To investigate the infection status and the immune effect of the highly pathogenic porcine reproductive and respiratory syndrome (HP-PRRS) from immunized pig populations of Panjin city in 2011, 2160 serum samples and 120 tissue specimens were collected from pig farms in Panjin city from January to December in 2011, and examined by enzyme linked immunosorbent assay (ELISA) and quantitative real-time PCR respectively. The results showed that there were not the highly pathogenic porcine reproductive and respiratory syndrome virus (HP-RRSV) in the tissues, the antibodies positive rate in Dawa,Panshan, Xinglongtai,Shuangtaizi was 82.2%, 81.1%,78.8%,81.1%, respectively; the antibodies positive rate in swine breeding farm,commercial pig farm,peasant household pigs was 90%,81.3%,75%, respectively. In summary, the immune status of HP-PRRS was fine, HP-PRRS did not break out in this year.

Key words: HP-PRRS; ELISA; quantitative real-time PCR