Abstract: The VP60 gene of rabbit haemorrhagic disease virus was cloned by RT-PCR and sequenced after purification, and then the amplied fragment was linked into prokaryotic expression vector pET28b(+). The recombinant plasmid was transformed into E.coli JM109. The identification was right, the recombinant plasmid was transformed into host strain E.coli Rosetta^TM,and induced by IPTG. The expressed protein of 62.0 ku was identified by SDS-PAGE and Western blotting.

Key words: RHDV; VP60; clone; expression; immunogenicity