应用RT-PCR检测猪传染性胃肠炎病毒的研究

Abstract

Abstract: According to N protein gene sequence of TGEV which was published in GenBank, designed and synthesized 1 pair of primers, which amplified fragment length of 730 bp, the detection mthods of TGEV by RT-PCR was established on the basis of the optimization of RT-PCR reaction conditions. This study established the TGEV detection methods, specific, sensitive and reliable for the diagnosis through clinical detection of diarrheal diseases and which will provide technical support for epidemiological studies on transmissible gastroenteritis virus.

Key words: transmissible gastroenteritis virus; RT-PCR; detection

CLC Number:

S858.28

ZHU Fen-hua. Study on Detecting Transmissible Gastroenteritis Virus by Reverse Transcription Polymerase Chain Reaction[J]. China Animal Husbandry & Veterinary Medicine, 2012, 39(4): 91-93.

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Study on Detecting Transmissible Gastroenteritis Virus by Reverse Transcription Polymerase Chain Reaction

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