›› 2012, Vol. 39 ›› Issue (4): 39-41.

• 生物技术 • Previous Articles     Next Articles

The Expression of MTNR1a Gene in Pituitary of Inner Mongolia Cashmere Goats

ZHENG Zhu-qing1,2, SHI Ling1,2, WANG Meng-yi3, WU Jing4, LIU Zhi-hong1,2, ZHANG Yan-jun1,2, SU Rui1,2, ZHAO Yan-hong1,2   

  1. 1. College of Animal Science, Inner Mongolia Agricultural University, Huhhot 010018, China;2. Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction,Huhhot 010018, China;3. Hulunbeier Institute of Environmental Science, Hailaer 021008,China;4. College of Food Science and Engineering, Inner Mongolia Agricultural University, Huhhot 010018,China
  • Received:2011-10-24 Revised:1900-01-01 Online:2012-04-20 Published:2012-04-20

Abstract: In the study , the total RNA was extracted from pituitary of Inner Mongolia cashmere goats. The primers were designed according to the published MTNR1a in similar species . The expression of melatonin receptor gene MTNR1a was detected by using RT-PCR and in situ hybridization in Inner Mongolia cashmere goats . The results showed that the expression of MTNR1a was successfully detected by using RT-PCR ,by using in situ hybridization futher defined the expression and distribution of MTNR1a. The results suggested that the pituitary is the target organ of the melatonin ,the hormone secreted by pituitary may play an important role in the effect of melatonin on seasonal breeding of cashmere goats.

Key words: MTNR1a; Inner Mongolia cashmere goats; RT-PCR; in situ hybridization; pituitary; expression

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