鸭肝炎病毒GD株的分离与RT-PCR鉴定分析

Abstract

Abstract: In order to complete a duck hepatitis virus (DHV) stain's isolation and sero-genotyping research, the suspected DHV liver from died duckling in Guangdong province was selected as sample, and then was carried on regression analysis, ELD₅₀ determination and duck embryo neutralization test after viral isolation, duck embryo inoculation and purification. The results showed that the serotype of the duck hepatitis virus was typeⅠ. According to RT-PCR detect and sequence analysis, it was also identified as genetype Ⅰ of DHV. Named the isolated virus strain as GD.

Key words: duck hepatitis virus; isolation; RT-PCR; identification analysis

CLC Number:

S852.65

HUANG Chang-li;WEI Chun-ya;ZHANG Chao-yi;YUAN Jing-le;LIU Zhi-bin;ZHANG Gui-hong. Isolation and Identification Analysis of Duck Hepatitis Virus GD Strain by RT-PCR[J]. , 2012, 39(2): 156-159.

[1]	WANG Dongjing, WU Jincuomu, ZENG Jiangyong. Isolation, Identification and Biological Characteristics Analysis of Clostridium perfringens Type A from Yaks [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 229-241.
[2]	DOU Junfeng, WANG Zui, LI Li, LU Qin, JIN Xinxin, LING Xiaochun, LUO Qingping, ZHAI Xinguo. Identification of Subgroups A, B and J Avian Leukosis Virus Co-infection and Sequence Analysis of gp85 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 302-311.
[3]	YANG Tingting, FENG Zijuan, HUANG Yi, ZHOU Honghong. Isolation, Identification and Biological Characteristics of Enterobacter hormaechei Phage [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 323-329.
[4]	ZHANG Lei, CHEN Liang, JIN Zhenhua, FENG Wanyu, MIAO Yan, LAN Shijie, QIN Pingwei, SHEN Sisi, LI Dan, ZHANG Bei, DONG Jiaqiang, HUANG Xuankai, LI Xuye, JIANG Botao, WANG Hongbao, SHI Tongrui. Isolation, Identification and Pathogenic Characteristics of One Pasteurella multocida Isolate from Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 347-356.
[5]	WANG Yaqin, ZONG Junlin, ZHANG Tao, GUAN Weijun. Isolation,Culture and Differentiation Potential of Bovine Adipose Mesenchymal Stem Cells [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3530-3540.
[6]	CHEN Changyi, LI Gen, HE Hongling, CHEN Yutong, HUANG Xuezhe, LUO Jun, GUO Xiaofeng. Isolation,Identification and Whole Genome Sequence Analysis of a Strain of Feline Calicivirus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3707-3718.
[7]	LI Tingting, LIU Zichao, ZHOU Yongshun, HE Ying, GENG Fanghao, CHEN Jieran, GAO Jianfeng. Effects of Two Treatment on Analysis of Mouse Dendritic Cells for Presentation of Brucella abortus Immunopeptidome in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3730-3739.
[8]	ZI Jiaxin, LIAN Yingjie, CHEN Minyi, LI Qiuxu, ZHANG Weize, LU Mengke, ZHANG Yangyang, QU Haoxuan, MA Jun, BAI Aiquan. Drug Resistance and Pathogenicity of 4 Strains of Streptococcus suis Type 2 in Guangdong [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3740-3751.
[9]	SUN Yue, WANG Qi, MAO Wei, WANG Bo, DONG Haiyan, FAN Hongliang, HAO Puguo, ZHAO Hongxia. Drug Resistance Analysis and Virulence Gene Detection of Escherichia coli Causing Calf Diarrhea in Inner Mongolia [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3811-3822.
[10]	WANG Dongjing, SU Zhonghua, YUAN Zhenjie, MA Hongcai, ZENG Jiangyong. Isolation,Identification and Biological Characteristics of Streptococcus Isolated from Tibetan Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3823-3832.
[11]	MA Jing, YANG Danjiao, WEI Longpin, GAN Daibing, LONG Zhixin, HE Yuqi, QING Ting, FAN Yandi, ZHOU Long. Isolation,Identification and Biological Characteristics Analysis of a Moraxella pluranimalium Strain from Tibetan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3275-3285.
[12]	YAN Han, TIAN Yangqing, ANG Yanfen, WANG Yayuan, ZHANG Xuefeng, YAN Yulin. Study on the Isolation, Identification and Culture Characteristics of Canine Hematopoietic Stem Cells [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2678-2687.
[13]	TANG Haibo, JIANG Jiajia, CHEN Fenglian, YANG Jinlan, BAI Anbin, LIU Jinfeng, QIN Shaomin, WU Jianmin. Isolation, Identification and Genomic Characterization Analysis of Parvovirus Isolate from Bamboo Rat [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2843-2853.
[14]	ZOU Minghao, MA Hongcai, WANG Bingyi, FAN Shijie, ZENG Jiangyong, WU Qingxia. Isolation, Identification and Drug Resistance Analysis of Staphylococcus chromogenes in Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 3017-3025.
[15]	LU Yukui, LIU Jiaqi, ZHONG Jiacheng, CHEN Jidang, ZHU Wanjun, ZHANG Yishan, ZHANG Jipei. Isolation,Identification and Pathogenicity Analysis of Avibacterium paragallinarum from Geese [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2562-2572.

Isolation and Identification Analysis of Duck Hepatitis Virus GD Strain by RT-PCR

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments