China Animal Husbandry & Veterinary Medicine ›› 2012, Vol. 39 ›› Issue (2): 121-124.

• 遗传繁育 • Previous Articles     Next Articles

Cloning and Sequence Analysis of the Promoter Region of Mongolian Sheep GDF11 Gene

HE Xiao-long1,2, LI Bei3, LIU Yong-bin1, WANG Feng1, TIAN Chun-ying1, RONG Wei-heng1   

  1. 1. Inner Mongolia Academy of Agriculture and Animal Science,Hohhot 010031,China;2. Inner Mongolia Research Center for Prataculture,Chinese Academy of Sciences,Hohhot 010031,China;3. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018,China
  • Received:2011-10-10 Revised:1900-01-01 Online:2012-02-20 Published:2012-02-20

Abstract: In order to analysis the relationship between growth differentiate factor 11 (GDF11) and the traits of more vertebras of Mongolian sheep, the gene promoter region was cloned firstly and analysised by the bioinformatics software. The results showed that the GDF11 gene promoter region length was 512 bp and bases proportion were A 10.55%, G 16.80%, T 31.84%, C 40.82%, the proportion of G+C was 57.62%. The results of bioinformatics analysis showed no CpG island and TATA box or CAAT box found in the Mongolian sheep GDF11 gene promoter region, but existed one transcription initiation site, 5 transcription factors: HSF2, HSF2, GATA-1, AML-1a, MZF1, and 5 motifs: EGF_1, CTCK_1, ANAPHYLATOXIN_1, VWFC_1, DEFENSIN. The research provided theoretical basis for the Mongolian sheep vertebra regulation mechanism by GDF11 gene.

Key words: Mongolian sheep; GDF11 gene; promoter; transcription factor

CLC Number: