1型鸭疫里氏杆菌的分离鉴定及其ompA基因B细胞表位预测

Abstract

Abstract: One bacterium strain was isolated from incidence duck in Guizhou,tested by physiological and biochemical characteristics,16S rRNA gene sequencing,recursive infection experiment and antibiotic sensitivity as RA serotype 1.According to the gene sequences in GenBank of RA,one pairs of specific primer was designed for amplifying the specific fragments of ompA.The specific bands of 1149 bp were amplified. Using DNAStar Protean software,the amino acid sequence encoded by ompA gene of RA was analyzed in secondary structure,hydrophilicity,flexibility and antigenic indexes to predict B cell epitopes,which was to lay the foundation for further study RA epitope vaccines and molecular diagnostic techniques to establish.

Key words: Riemerella anatipestifer; ompA gene; B cell epitope prediction

CLC Number:

YU Bo, XU Jing-e, WANG Xuan, TAN Shi-wen. Isolation and Identification of Riemerella anatipestifer Serotype 1 and Prediction of Epitopes on B Cell of ompA Gene[J]. China Animal Husbandry & Veterinary Medicine, 2012, 39(12): 51-55.

References

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Isolation and Identification of Riemerella anatipestifer Serotype 1 and Prediction of Epitopes on B Cell of ompA Gene

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