肉碱棕榈酰转移酶基因在绵羊和山羊不同肌肉组织中的表达分析

Abstract

Abstract: The author performed the comparative analysis of carnitine palmitoyl transferase(CPT)gene in different tissues from sheep and goats by quantification PCR. The results showed that CPT1A mRNA expression in sheep liver and spleen was higher than CPT1B and CPT2 expression in sheep liver and spleen. Expression of CPT1B mRNA in the external oblique muscle of sheep was higher than CPT1A, CPT2 mRNA expression in the external oblique muscle of sheep. CPT1A mRNA expressed in goat spleen was higher than the expression of CPT1B and CPT2 mRNA in goat spleen. CPT1B mRNA in goats hind legs, biceps femoris and external oblique muscle were higher than CPT1A, CPT2 mRNA in goat hind shares two expression in the biceps. CPT1A mRNA expression in the liver of sheep was significantly higher than the expression of CPT1A mRNA in goats (P＜0.05). CPT1A, CPT1B, CPT2 gene expression in sheep heart, spleen, lumbar major muscle was significantly higher than the expression in goat(P＜0.05), the CPT1A, CPT1B, CPT2 gene expression in the hind legs in goat was significant higher than the amount of expression in sheep(P＜0.05). CPT family gene expression in the organization of sheep, goat differences might be associated with each gene expression patterns in different types of muscle tissue fiber content of fat deposition and other factors.

Key words: sheep; goat; carnitine palmitoyl transferase; skeletal muscle

CLC Number:

Q786

YU Bao-li, GAO Ai-qin, LI Jin-quan, ZHANG Wen-guang. Expression Analysis of CPT Gene in Different Tissues from Sheep and Goat[J]. , 2012, 39(10): 1-6.

References

1 毛红霞. L-肉碱对肉鸡生长、胴体组成和肉质的影响及其作用机理探讨.杭州:浙江大学,2001.
2 王镜岩,朱圣庚,徐长法,等. 生物化学(下册)[M]. 第3版. 北京:高等教育出版社,2002.
3 王晓方,常文环,刘国华,等. 畜禽肌肉肌苷酸研究进展[J]. 中国畜牧兽医,2012,39(5): 221~225.
4 冯宇,郭长江,等. 肉碱对高脂饲料喂养的半饥饿大鼠脂肪代谢的改善作用[J]. 中国病理生理杂志,2000,16(12):1313~1316.
5 乔永. 湖羊羔羊不同部位肌肉肌内脂肪沉积相关基因表达的发育性变化研究. 南京:南京农业大学,2007.
6 吴建平. 不同肉羊品种体脂脂肪酸遗传变异性及其特性的研究. 兰州:甘肃农业大学,2000.
7 吴成福,江世贵,林黑着,等. 饲料中L-肉碱对斑节对虾肌肉中肌苷酸含量的影响[J].上海水产大学学报,2008,17(1):72~76.
8 李家胜,陈民利. 高效液相色谱测定畜禽肌肉中的肌苷酸含量[J]. 浙江农业大学学报,1998,24(3):295~296.
9 张海艳,于太永,等. 肌苷酸形成机理及其含量影响因素浅析[J].中国农业科技导报,2004,6(3):17~21.
10 张艳芳. CPT1基因对猪脂肪沉积的影响极其调控机制的研究.杭州:浙江大学,2010.
11 陈国宏,李慧芳,等. 泰和乌骨鸡肌肉肌苷酸含量变化规律及其遗传力估测[J].扬州大学学报,2002,23(2):29~32.
12 罗桂芬,孙世铎,陈继兰,等. 肉类风味物质:肌苷酸[J].中国家禽,2004,26(3):41~43.
13 郝称莉. 湖羊肌肉肌内脂肪相关基因表达的发育性变化及与肌内脂肪含量的相关性研究.南京:南京农业大学,2008.
14 章世元,王俊丽,俞路,等. L-肉碱对异育银卿生长性能及肌肉品质的影响[J].中国饲料,2009,3:33~36.
15 曾勇庆,孙玉民,张万福,等. 莱芜猪肌肉组织学特性与肉质关系的研究[J].畜牧兽医学报,1998,29(6): 486~492.
16 蒋福虎,冯旭芳,李蒙召,等. 羊肉肌纤维生长规律的观察[J].山西农业大学学报,1996,16(4): 386~388.
17 Barker D L,Sell J L. Dietary carnitine did not influence performance and carcass composition of broiler chickens and young turkeys fed low or high-diets[J]. Poul Sci,1994,73(2):281~287.
18 Brien P J,Shen H,McCutcheon L J. Rapid,simple and sensitivemicroassay for skeletal and cardiac muscle myoglobin and hemoglobin:use in various animals indicates functional role of myohemoproteins [J]. Mol Cell Biochem,1992,112:45~52.
19 Hoppel C L,Saul M G. Carnitine metabolism in normal-weight and obese human subjects during fasting[J].Am J Physiol,1980, 238:E409~E415.
20 Mc Garry J D,Brown N F. The mitochondrial carnitine palmitoyltransferase system from concept to molecular analysis. Eur J Biochem,1997,244(1):1~14.

[1]	WANG Saiqiao, ZHAO Lu, ZHAI Zhenhan, ZHANG Binglei, JIA Wanhang, WANG Yuqin. Tissue Expression and Bioinformatics Analysis of miR-449a/b Precursor Sequences in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2175-2184.
[2]	YANG Guitao, MA Jideng, LI Xuewei, GE Liangpeng, ZHANG Jinwei. Research Advance on Regulation of Skeletal Muscle Physiology Function by Short Chain Fatty Acids [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2286-2295.
[3]	LYU Chunrong, LIANG Jiachong, SHAO Qingyong, WU Guoquan, ZHANG Yumei, QUAN Guobo. Effects of Sodium Pyruvate on the Quality of Frozen Goat Sperm [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2363-2369.
[4]	LIU Jinyu, ZHANG Yang, MA Xumei, ZHOU Qian, ZHOU Yang, ZHANG Chunxiang, SHI Lei, REN Youshe. Effects of Glutathione on Cryopreservation of Sheep Semen [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2388-2394.
[5]	WU Zhijuan, CHAI Zhixin, WANG Jikun, WANG Jiabo, ZHONG Jincheng, XIN Jinwei. Expression Analysis of Specific Matrix Metalloproteinases and Functionally Related Genes of Skeletal Muscles in Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1774-1784.
[6]	ZHANG Huaqiang, WANG Pinsheng, LI Xiao, WEI Panpeng, YAN Lingmin, JIANG Bingbing, WANG Zhaogui, WANG Xuefang, ZENG Huanqing, FAN Yi, GAO Zhenglong, WANG Xuebing. Effects of Fermented Hybrid Broussonetia papyrifera on Growth Performance, Meat Quality,Digestive Enzyme Activity and Intestinal Flora Structure of Hu Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1876-1887.
[7]	ZHAO Qianming, QI Meng, XU Zeli, GONG Pihong, LU Chenyang, PEI Zhiyang, JIAN Fuchun, WANG Jiandong, JING Bo, NING Changsheng. Investigation of Intestinal Parasites Occurrence in Sheep and Environmental Contamination in Large-scale Housing Farms [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2156-2165.
[8]	SONG Xingchao, MENG Jinzhu, ZHAO Yuanyuan, WU Zhenyang, AN Qingming. Cloning and Bioinformatics Analysis of MyoG Gene Promoter Region Sequence in Guizhou White Goat [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 893-903.
[9]	CUI Shuang, Wurlig, Alder, XIAO Min, WANG Jianqiu, GONG Jian. Effects of Yeast Selenium Supplementation on Gastrointestinal Microflora in Sheep Under Grazing Conditions [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 952-965.
[10]	ZHEN Zhen, WANG Mei, WANG Yimin, HU Debao, ZHANG Linlin, LI Xin, GUO Yiwen, GUO Hong, DING Xiangbin. Effect of RNA Methylation Transfer Enzyme METTL3 on the Proliferation and Myogenic Differentiation of Bovine Skeletal Muscle Satellite Cells [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1025-1036.
[11]	Sachula, WU Tiecheng, MA Yuejun, HE Yunmei, ZHU Lixian, HE Tuoya, WU Yuping, LIU Bin. Polymorphisms of Four Polyembryony Related Genes and Its Association Analysis with Litter Size in Cashmere Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1037-1047.
[12]	ZHANG Yongjie, SUI Zhiyuan, ZHANG Zhishuai, WANG Chenguang, LI Xiaojun, XING Feng. Cloning and Bioinformatics Analysis of NPTX1 Gene and Its Tissues Expression at Puberty in Dolang Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1048-1058.
[13]	WEI Yirong, ZHENG Zihua, ZHANG Sanbao, SONG Ying, JIANG Haiyu, SUN Wenyue, CHENG Pengfei, LIU Yufan, ZOU Jianwei, HUANG Yanna, PAN Yan, JIANG Qinyang. Cloning,Bioinformatics Analysis and Tissue Expression of UCP1 Gene in Nubian Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 440-450.
[14]	ZHANG Mingliang, ZHANG Hailong, MA Shipeng, WANG Cailian, LANG Xia. Effects of Zanthoxylum bungeanum Essential Oil on Production Performance and Gastrointestinal Tissue Structure of Small-tail Han Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 556-563.
[15]	LI Chenglan, GUO Tingting, YUE Yaojing. Research Advances on Genomic Selection and Its Application in Sheep Breeding [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 616-625.

Expression Analysis of CPT Gene in Different Tissues from Sheep and Goat

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments